Journal of Applied Biomedicine, 2022 (vol. 20), issue 4
Original Research Article
Neuropsychological tests and prediction of dementia in association with the degree of carotid stenosis
Ondřej Machaczka, David ©koloudík, Jana Janoutová, Martin Roubec, Eva Reiterová, Martina Kovalová, Anna Zatloukalová, Petr Ambroz, Vladimír Janout
J Appl Biomed 20:115-123, 2022 | DOI: 10.32725/jab.2022.018 
This study constitutes a cross sectional analysis of the association between cognitive impairment defined by neuropsychological tests and carotid stenosis. The main objective was to compare the results of the Mini-Mental State Examination (MMSE) and Addenbrooke's Cognitive Examination-Revised (ACE-R) with regard to the degree of carotid stenosis. The sample comprised 744 patients who underwent a carotid duplex ultrasound and cognitive function testing (by ACE-R and MMSE). A multivariable analysis of potential confounding factors was completed. The significance of the different number of positive (MMSE ≤ 27, ACE-R ≤ 88) and negative (MMSE...
Topical application of indigo-plant leaves extract enhances healing of skin lesion in an excision wound model in rats
Mohamed A. Saleh, Ahmed A. Shabaan, Michel May, Youssif M. Ali
J Appl Biomed 20:124-129, 2022 | DOI: 10.32725/jab.2022.014
Objectives: This study aims to evaluate the pharmacological role of indigo extract in accelerating the wound healing in a rat model. Methods: Female Sprague-Dawley rats were anesthetized with ketamine (30 mg/kg, i.p.) and the full thickness of the marked skin was then cut carefully and wounds were left undressed. Indigo extract (5%) in PBS was applied topically twice daily until healing was complete. A control group of rats was treated with povidone-iodide (Betadine®). Rats treated with phosphate buffer saline were used as a negative control group. The rate of wound healing was assessed daily. Histopathological examination of skin sections were...
Exenatide prevents statin-related LDL receptor increase and improves insulin secretion in pancreatic beta cells (1.1E7) in a protein kinase A-dependent manner
Łukasz Bułdak, Grzegorz Machnik, Estera Skudrzyk, Aleksandra Bołdys, Mateusz Maligłówka, Michał Kosowski, Marcin Basiak, Rafał Jakub Bułdak, Bogusław Okopień
J Appl Biomed 20:130-140, 2022 | DOI: 10.32725/jab.2022.015
Statins are primary drugs in the treatment of hyperlipidemias. This group of drugs is known for its beneficial pleiotropic effects (e.g., reduction of inflammatory state). However, a growing body of evidence suggests its diabetogenic properties. The culpable mechanism is not completely understood and might be related to the damage to pancreatic beta cells. Therefore, we conceived an in vitro study to explore the impact of atorvastatin on pancreatic islet beta cells line (1.1.E7). We evaluated the influence on viability, insulin, low-density lipoprotein (LDL) receptor, and proprotein convertase subtilisin/kexin type 9 (PCSK9) expression. A significant...
Assessment of plasma catecholamines in patients with dysmetabolic iron overload syndrome
Hany William Z. Hanna, Heba N Baz, Lika'a Fasih Y. Al-Kzayer, Hemmat E El Haddad, Fatma El-Mougy
J Appl Biomed 20:141-145, 2022 | DOI: 10.32725/jab.2022.016
Background: Dysmetabolic iron overload syndrome (DIOS) is characterized by hyperferritinemia and normal transferrin saturation level with components of metabolic syndrome (MS). Among cases of MS, we determined those with DIOS and their characterizations, then we evaluated the association between plasma catecholamines status and hypertension in DIOS. Methods: We compared 101 hypertensive patients with 50 healthy participants (control group). Iron (iron, transferrin, and ferritin), insulin, and plasma catecholamine (adrenaline, noradrenaline, and dopamine), profiles were measured for both groups. Homeostasis model assessment of insulin resistance index...
ADIPOQ-rs2241766 polymorphism is associated with changes in cholesterol levels of Mexican adolescents
Rafael Baltazar Reyes Leon-Cachon, Mauricio Andres Salinas-Santander, Daniela Alejandra Aguilar-Tamez, Paola MarianaValdez-Ortiz, Clara Patricia Rios-Ibarra, Ana Cecilia Cepeda-Nieto, Victor de Jesus Suarez-Valencia, Jesus Antonio Morlett-Chavez
J Appl Biomed 20:146-153, 2022 | DOI: 10.32725/jab.2022.017
Background: The ADIPOQ gene encodes a fat-derived protein hormone with a preponderant role in the homeostasis of glucose and fatty acids. However, previous association studies between ADIPOQ genetic variants and metabolic disorders have shown controversial results. In this study, we evaluated the effect of the ADIPOQ-rs2241766 polymorphism on diverse biochemical parameters (i.e., insulin resistance, atherogenic index, overweight and obesity) in an adolescent population from Mexico. Methods: A cross-sectional study with convenience sampling was carried out in 356 adolescents from Northern Mexico. They were classified by sex...
Review Article
The use of periodontal membranes in the field of periodontology: spotlight on collagen membranes
Hanène Ayari
J Appl Biomed 20:154-162, 2022 | DOI: 10.32725/jab.2022.020
Periodontal regenerative techniques are performed to accomplish the restitution of soft and hard teeth-supporting tissues that have been lost due to trauma or inflammatory disease. Periodontal membranes are used for these techniques to provide support and a framework for cell growth and tissue regeneration. They act as a temporary and selective barrier to cell proliferation. Easy clinical handling, biomechanical specifications, high biocompatibility, cell-occlusivity, and satisfactory bioresorption rate are essential properties a membrane needs to be effective. The creation and maintenance of a secluded space is also a fundamental rule in periodontal...