Journal of Applied Biomedicine, 2024 (vol. 22), issue 3
Original Research Article
Astragaloside IV confronts amyloid-beta-induced astrocyte senescence via hsp90aa1
Xia Yan, Rongxiang Zeng, Yajun Cao
J Appl Biomed 22:129-135, 2024 | DOI: 10.32725/jab.2024.015 
Cell senescence is intensively related to aging and neurodegenerative diseases. This study aimed to explore the effect and targets of Astragaloside IV against amyloid-beta-induced astrocyte senescence. Oligomerized amyloid-beta was prepared to culture with human astrocytes. The effects of Astragaloside IV were assessed based on SA-β-gal staining analysis, senescence markers (p53, p16INK4, and p21WAF1), neurotrophic growth factor levels (qRT-PCR), and cell proliferation (CCK-8 kit). The targets for Astragaloside IV were predicted, and hsp90aa1 protein was verified using molecular docking. After hsp90aa1 overexpression, the effects of Astragaloside...
Anti-NMDAR1 antibody impairs dendritic branching in immature cultured neurons
Pascal Jorratt, Aneta Petrušková
J Appl Biomed 22:136-140, 2024 | DOI: 10.32725/jab.2024.019
Anti-N-methyl D-aspartate receptor (anti-NMDAR) encephalitis is an autoimmune disorder characterized by IgG antibodies targeting NMDAR. The prevalence is remarkably higher in women and some develop the condition during pregnancy. While immunotherapies have shown good outcomes for pregnant mothers and their infants, the impact on early neurodevelopment remains elusive. This study investigates the effects of anti-NMDAR antibody on the development of primary cortical cultures. Anti-NMDAR antibody was administered to the cultures at day in vitro 5 for the following 5 days to assess dendritic branching and arbor complexity, and at day in...
Salivary glands - a new site of Helicobacter pylori occurrence
Jan Rotnágl, Jiří Hložek, Richard Holý, Emil Pavlík, David Kalfeřt, Jaromír Astl
J Appl Biomed 22:141-148, 2024 | DOI: 10.32725/jab.2024.018
Objective: The role of Helicobacter pylori (Hp) in the pathological processes of the gastric mucosa is well understood. Decreasing trend in successful eradication of HP from the stomach was observed in last years. This lack of succes is mainly caused by increasing ATB resistance. Nevertheless other possible causes of this phenomenon are being explored. Thus, many studies have focused on the search for extragastric reservoirs as potential sources of persistence or reinfection after successful Hp eradication. The pathological potential of Hp at these localities has also been studied. Methods: Our study aimed to determine the presence of Hp inside...
Olfactory event-related potentials (OERPs) and trigeminal event-related potentials (TERPs) in subjects after Covid-19 infection: single-center prospective study
Richard Holý, David Kalfeřt, Libor Vašina, Oleksii Vorobiov, Petra Dytrych, Karla Janoušková, Eva Augste, Shahriar Kashiri, Nikola Pastorková, Kristýna Mamiňák, Jiří Hložek, Daniel Kovář, Jan Vodička, Jaromír Astl
J Appl Biomed 22:149-154, 2024 | DOI: 10.32725/jab.2024.020
Objectives: Olfactory dysfunction (OD) is a common symptom associated with Covid-19. During the Covid-19 pandemic, the importance of psychophysical olfactory tests and electrophysiological olfactory assessment increased. The purpose of the study was to analyze the psychophysical olfactory tests and the post-covid curves of olfactory event-related potentials (OERPs) and trigeminal event-related potentials (TERPs). Methods: The prospective study included 98 subjects (62 females / 36 males). The average age was 42 years (range 21-84 years). Group I (n = 77) contained participants who had been infected with Covid-19. They were enrolled in the study...
In vitro biological activities of Calamintha nepeta L. aqueous extracts
Nadia Salhi, Dorien Deluyker, Virginie Bito, Abdelhamid Zaid, Lhoussaine El Rhaffari
J Appl Biomed 22:155-163, 2024 | DOI: 10.32725/jab.2024.017
Aim: This study aimed to investigate the phenolic composition, antioxidant capacity, and toxicity of aqueous extracts of Calamintha nepeta L. leaves and their potential vasorelaxant effects. Methods: Aqueous extracts of Calamintha nepeta L. were prepared by three extraction methods: decoction, infusion, and maceration. The total phenolic contents of the extracts and their antioxidant properties were investigated. The toxicity was evaluated by Artemia salina lethality bioassay. The decoction extract was analyzed by HPLC for its chemical profile and was also used to evaluate the vasorelaxant effect on thoracic aortic rings isolated...
Review Article
Today's cancer research and treatment - highly sophisticated and molecularly targeted, yet firmly bolstered in the classical theories
Thomas W. Grunt
J Appl Biomed 22:123-128, 2024 | DOI: 10.32725/jab.2024.016
Cancer research is linked to modern life-sciences, encompassing achievements in virology, yeast-biology, molecular-biology, genetics, systems-biology, bioinformatics, and so on. With these fascinating developments, it's easy to overlook that the fundamental theories and treatment strategies were established in the early 20th century and have remained valid ever since. Therefore, tribute must be paid to the founders of the field. The main hypotheses on carcinogenesis, the genetic model and the metabolic model, and the concept of cancer-treatment with cytotoxic, targeted or metabolic drugs were proposed more than 100 years ago by great minds such as...