Journal of Applied Biomedicine, 2025 (vol. 23), issue 1

Original Research Article

Chemical composition and anticancer activity of Psychotria montana on MCF7 breast cancer cells: insights from in vitro (2D & 3D) studies and in silico analysis

Van Hung Hoang, Thi Kieu Oanh Nguyen, Phu Hung Nguyen, Thi Thanh Huong Le, Viet Hoang

J Appl Biomed 23:12-25, 2025 | DOI: 10.32725/jab.2025.002  

Aim: This study aimed to investigate the phytochemical composition of Psychotria montana extract (PME) and evaluate its inhibitory effects on MCF7 breast cancer cells. Methods: The chemical composition of PME was analyzed using UPLC-QToF-MS. The effects of PME on cell proliferation were evaluated using the MTT assay. Flow cytometry was used for cell cycle and apoptosis analysis. The effects of PME on the transcription of cell cycle control genes were assessed using real-time PCR. Results: UPLC-QToF-MS analysis revealed major compounds of PME, including terpenoids and flavonoids, with the potential to inhibit proliferation, migration, and induce...

Changes in glucose-related parameters according to LDL-cholesterol concentration ranges in non-diabetic patients

Vladimír Kron, Miroslav Verner, Pavel Smetana, Radka Vrzalová, Eliška Friebergerová, Daniel Martiník, Dagmar Horáková, Ladislav Štěpánek, Lidmila Hamplová, Kvetoslava Kotrbová

J Appl Biomed 23:26-35, 2025 | DOI: 10.32725/jab.2025.005  

The study focused on the changes in C-peptide, glycemia, insulin concentration, and insulin resistance according to LDL-cholesterol concentration ranges. The metabolic profile of individuals in the Czech Republic (n = 1840) was classified by quartiles of LDL-cholesterol into four groups with the following ranges: 0.46-2.45 (n = 445), 2.46-3.00 (n = 474), 3.01-3.59 (n = 459), and 3.60-7.18 mmol/l (n = 462). The level of glucose, C-peptide, insulin, and area of parameters during OGTT and HOMA IR were compared with a relevant LDL-cholesterol range. The evaluation involved correlations between LDL-cholesterol and the...

Distribution of human leukocyte antigen B27 (HLA-B27) in Slovak patients

Alexandra Pôbišová, Andrea Kollárová, Vladimír Langraf, František Strejček, Ramona Babosová

J Appl Biomed 23:36-44, 2025 | DOI: 10.32725/jab.2025.004  

Background and objectives: HLA-B27 is a genetic marker associated with spondyloarthropathies, particularly ankylosing spondylitis and axial spondyloarthritis. While its prevalence varies across populations, no data exist for Slovak patients. This study aimed to determine HLA-B27 prevalence in Slovak patients with suspected spondyloarthropathies and assess differences by sex and age. Methods: A retrospective cohort of 1,614 patients (888 females and 726 males) was analyzed for HLA-B27 status (positive/negative) using reverse hybridisation (HLA-B27 StripAssay). Statistical analyses included Pearson's Chi-square test and non-parametric Mann-Whitney U...

Seleno-chitooligosaccharide-induced modulation of intestinal barrier function: Role of inflammatory cytokines, tight junction proteins, and gut microbiota in mice

Zhuo-Hong He, Ye Jin, Die Chen, Hui-Xin Zheng, Jia-Er Xiang, Yong-Jun Jiang, Zheng-Shun Wen

J Appl Biomed 23:45-55, 2025 | DOI: 10.32725/jab.2025.003  

This study aimed to explore the function of Seleno-chitooligosaccharide (SOA) on the intestinal barrier through regulation of inflammatory cytokines, tight junction protein, and gut microbiota in mice. The results of ELISA assay demonstrated that SOA significantly increased the levels of IL-2, IL-10, and IFN-γ in serum and ileum. Meanwhile, SOA increased the levels of IL-4 in the ileum (p < 0.05). In addition, Diamine Oxidase (DAO) concentration was decreased in ileum by SOA treatments (p < 0.05). The administration of SOA significantly upregulated the expression of ZO-1 and Occludin in the ileum (p < 0.05). By 16S...

Review Article

Systematic review of antibacterial potential in calcium oxide and silicon oxide nanoparticles for clinical and environmental infection control

Hend Algadi, Mohammed Abdelfatah Alhoot, Laith A. Yaaqoob

J Appl Biomed 23:1-11, 2025 | DOI: 10.32725/jab.2025.001  

A substantial threat to worldwide health, the proliferation of antibiotic-resistant bacteria compels researchers to seek innovative antibacterial substances. This systematic review assesses the role of nanoparticles, particularly Calcium oxide and Silicon oxide nanoparticles, in infection control. The article examines the mechanisms by which these nanoparticles act against various bacteria and evaluates their potential as novel agents in infection control strategies. A systematic literature search from 2015 to 2024 encompassing Web of Science, PubMed, Wiley, Science Direct, and Google Scholar, yielded 70 publications meeting the review criteria. This...