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- 2.0 – 2-year Impact Factor
- 1.8 – 5-year Impact Factor
- EISSN 1214-0287 • ISSN 1214-021X
- double-blind evaluation of manuscript by independent international experts
- fast on-line publication of accepted manuscripts
- open access
- published from 2003 – freely available (since 2019 + 2003-2013), published by Elsevier 2014-2018
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Recent achievements
Two our publications got the Award of the Czech Society of Otorhinolaryngology and Head and Neck Surgery for the best publication in 2024
Latest articles
Results 61 to 71 of 71:
The hepatoprotective effect of Sophora viciifolia fruit extract against acetaminophen-induced liver injury in miceOriginal Research Article
Shanshan Qi, Beibei Lin, Sanqiao Wu, Hao Hao, Hongxin Zheng, Xiang Liu, Xiaoying Zhang, Lijuan Yue, Chen Chen
J Appl Biomed 21:91-98, 2023 | DOI: 10.32725/jab.2023.008 
This research demonstrated the protective effect and possible mechanism of the Sophora viciifolia extract (SVE) against acetaminophen-induced liver injury in mice. The levels of ALT and AST in the serum and antioxidant enzyme activity in the liver were measured. We used immunohistochemistry to detect CYP2E1, Nrf2, and Keap1 protein expression in the liver. The mRNA expression in the liver of TNF-α, NF-κB, and IL-6, Nrf2 and its downstream genes HO-1 and GCLC were measured by qRT-PCR. We found that SVE could decrease the ALT and AST levels, promote the activities of SOD, CAT, GSH-Px,...
Antioxidant action of xanthine oxidase inhibitor febuxostat protects the liver and blood vasculature in SHRSP5/Dmcr ratsOriginal Research Article
Mai Kakimoto, Moe Fujii, Ikumi Sato, Koki Honma, Hinako Nakayama, Sora Kirihara, Taketo Fukuoka, Shang Ran, Satoshi Hirohata, Kazuya Kitamori, Shusei Yamamoto, Shogo Watanabe
J Appl Biomed 21:80-90, 2023 | DOI: 10.32725/jab.2023.009
Background: Xanthine oxidase (XO) generates reactive oxygen species during uric acid production. Therefore, XO inhibitors, which suppress oxidative stress, may effectively treat non-alcoholic steatohepatitis (NASH) and atherosclerosis via uric acid reduction. In this study, we examined the antioxidant effect of the XO inhibitor febuxostat on NASH and atherosclerosis in stroke-prone spontaneously hypertensive 5 (SHRSP5/Dmcr) rats. Methods: SHRSP5/Dmcr rats were divided into three groups: SHRSP5/Dmcr + high-fat and high-cholesterol (HFC) diet [control group, n = 5], SHRSP5/Dmcr + HFC diet + 10% fructose (40 ml/day) [fructose group, n =...
In vitro study of Nigella sativa and thymoquinone activity on endothelial activation and monocyte adhesionOriginal Research Article
Al'Aina Yuhainis Firus Khan, Fahmi Mohtar, Thuhairah Abdul Rahman, Suhaila Abdul Muid, Gabriele Ruth Anisah Froemming, Hapizah Nawawi
J Appl Biomed 21:73-79, 2023 | DOI: 10.32725/jab.2023.006
Introduction: Thymoquinone (TQ) is one of the bioactive compounds in Nigella sativa (NS). Also known as black seeds/cumin, it has been postulated to possess anti-atherogenic properties. However, research on the effects of NS oil (NSO) and TQ on atherogenesis remain scarce. The aim of this study is to determine gene and protein expression of Intercellular Adhesion Molecule-1 (ICAM-1), Vascular Cell Adhesion Molecule-1 (VCAM-1), and Endothelial-eukocyte adhesion molecule (E-selectin) in Human Coronary Artery Endothelial Cells (HCAECs). Methods: HCAECs were stimulated for 24 hours (h) with 200 µg/ml of Lipopolysaccharides (LPS) and different...
Sequential hybrid ablation versus surgical CryoMaze alone for treatment of atrial fibrillation (SurHyb Trial): a protocol of the multicentre randomized controlled trialOriginal Research Article
Alan Bulava, Aleš Mokráček, Dan Wichterle, Petr Budera, Pavel Osmančík, Petr Kačer, Linda Vetešková, Petr Němec, Tomáš Skála, Petr Šantavý, Jan Chovančík, Piotr Branny, Vitalii Rizov, Miroslav Kolesár, Marian Rybář
J Appl Biomed 21:67-72, 2023 | DOI: 10.32725/jab.2023.007
Background: Atrial fibrillation is common in patients with structural heart disease who are undergoing cardiac surgery. Surgical CryoMaze has been shown to be an effective treatment in several trials, but success rates have varied considerably, between 47-95%. The sequential hybrid approach, combining surgical CryoMaze followed by radiofrequency catheter ablation, can achieve high freedom from atrial arrhythmias. However, in patients with concomitant surgical atrial fibrillation treatment, data comparing the hybrid approach to CryoMaze alone are lacking. Methods: The SurHyb study was designed as a prospective, open-label, multicentre randomized trial....
Causality assessment of adverse drug reaction: A narrative review to find the most exhaustive and easy-to-use tool in post-authorization settingsReview Article
Pallavi Pradhan, Maude Lavallée, Samuel Akinola, Fernanda Raphael Escobar Gimenes, Anick Bérard, Julie Méthot, Marie-Eve Piché, Jennifer Midiani Gonella, Lyne Cloutier, Jacinthe Leclerc
J Appl Biomed 21:59-66, 2023 | DOI: 10.32725/jab.2023.010
Background: The core motive of pharmacovigilance is the detection and prevention of adverse drug reactions (ADRs), to improve the risk-benefit balance of the drug. However, the causality assessment of ADRs remains a major challenge among clinicians, and none of the available tools of causality assessment used for assessing ADRs have been universally accepted. Objective: To provide an up-to-date overview of the different causality assessment tools. Methods: We conducted electronic searches in MEDLINE, EMBASE, and the Cochrane database. The eligibility of each tool was screened by three reviewers. Each eligible tool was then scrutinized for its domains...
Effects of oleanolic acid on hair growth in mouse dorsal skin mediated via regulation of inflammatory cytokinesOriginal Research Article
Bing Zhang, Wenyun Zhang, Jianfang Luo, Jian He, Xiaomin Zheng, Siyang Zhu, Baoshan Rong, Yong Ai, Lanyue Zhang, Tinggang He
J Appl Biomed 21:48-57, 2023 | DOI: 10.32725/jab.2023.003
Oleanolic acid (OA) is a pentacyclic triterpenoid with favourable physiological activity. It is widely distributed in more than 200 species of plants. OA has garnered significant interest because of its potential biological activities, such as antioxidant, bacteriostatic, and hair growth-promoting effects. To study the effect of OA on hair growth and related mechanisms, we investigated hair growth in mice with testosterone-induced androgenetic alopecia (AGA) that were treated with three different concentrations of OA. The antioxidant, bacteriostatic, and cytotoxic effects of OA were evaluated. We found that mice with testosterone-induced AGA...
Amaryllidaceae alkaloids in skin cancer management: Photoprotective effect on human keratinocytes and anti-proliferative activity in melanoma cellsOriginal Research Article
Carol Castañeda, Karent Bravo, Natalie Cortés, Janeth Bedoya, Warley de S. Borges, Jaume Bastida, Edison Osorio
J Appl Biomed 21:36-47, 2023 | DOI: 10.32725/jab.2023.004
Skin cancer has high rates of mortality and therapeutic failure. In this study, to develop a multi-agent strategy for skin cancer management, the selective cytotoxicity of several alkaloid fractions and pure alkaloids isolated from Amaryllidaceae species was evaluated in melanoma cells. In addition, UVB-stimulated keratinocytes (HaCaT) were exposed to seven alkaloid fractions characterized by GC-MS, and the production of intracellular reactive oxygen species (ROS) and IL-6, were measured to evaluate their photoprotection effects. The Eucharis caucana (bulb) alkaloid fraction (20 μg/ml) had a clear effect on the viability of melanoma cells,...
As a novel anticancer candidate, ether extract of Dendrobium nobile overstimulates cellular protein biosynthesis to induce cell stress and autophagyOriginal Research Article
Ruoxi Zhao, Shigang Zheng, Ying Li, Xueqin Zhang, Dan Rao, Ze Chun, Yadong Hu
J Appl Biomed 21:23-35, 2023 | DOI: 10.32725/jab.2022.019
Increasing data has confirmed the potential anticancer properties of Dendrobium, a traditional Chinese herb. However, most anticancer compositions from the plant of Dendrobium were usually extracted by high polar solvent, while weak polar compositions with excellent anticancer activity remained largely unexplored. In this study, the differences between ether extract and ethanol extract of Dendrobium nobile Lindl. on chemical components and anticancer activities were investigated, as well as the anticancer mechanisms among different extracts. The results demonstrated that the ether extract exhibited a stronger anticancer effect...
hKLK alleviates myocardial fibrosis in mice with viral myocarditisOriginal Research Article
Youfa Qin, Xiaomei Ye, Ye Luo, Luting Peng, Guanghui Zhou, Yongkun Zhu, Chunyu Pan
J Appl Biomed 21:15-22, 2023 | DOI: 10.32725/jab.2023.005
Myocardial fibrosis is the most serious complication of viral myocarditis (VMC). This study aimed to investigate the therapeutic benefits and underlying mechanisms of lentivirus-mediated human tissue kallikrein gene transfer in myocardial fibrosis in VMC mice. We established VMC mouse model via intraperitoneal injection with Coxsackie B3 virus. The effect was then assessed after treatment with vehicle, the empty lentiviral vectors (EZ.null), and the vectors expressing hKLK1 (EZ.hKLK1) via tail vein injection for 30 days, respectively. The results showed that administering EZ.hKLK1 successfully induced hKLK1 overexpression in mouse heart. Compared with...
Comparison of cardiocerebrovascular disease incidence between angiotensin converting enzyme inhibitor and angiotensin receptor blocker users in a real-world cohortOriginal Research Article
Suehyun Lee, Hyunah Kim, Hyeon Woo Yim, Hun-Sung Kim, Ju Han Kim
J Appl Biomed 21:7-14, 2023 | DOI: 10.32725/jab.2023.002
Background: Both angiotensin-converting enzyme inhibitors (ACEIs) and angiotensin II receptor blockers (ARBs) are known to be effective in managing cardiovascular diseases, but more evidence supports the use of an ACEI. This study investigated the difference in cardiovascular disease incidence between relatively low-compliance ACEIs and high-compliance ARBs in the clinical setting. Methods: Patients who were first prescribed ACEIs or ARBs at two tertiary university hospitals in Korea were observed in this retrospective cohort study for the incidence of heart failure, angina, acute myocardial infarction, cerebrovascular disease, ischemic heart disease,...
Closed reduction and minimally invasive screw osteosynthesis of Pipkin femoral head fracturesOriginal Research Article
Martin Kloub *, Karel Holub, Marek Peml, Jiří Urban, Pavel Látal
J Appl Biomed 21:1-6, 2023 | DOI: 10.32725/jab.2023.001
Background: Femoral posterior hip dislocation with associated femoral head fractures (Pipkin fractures) are rare high-energy injuries. Published treatment modalities involve conservative treatment, head fragment resection, open reduction and internal fixation, and total hip replacement. The experience with mini-invasive screw osteosynthesis of these fractures is the main focus of our study. Methods: Seven Pipkin fractures (five Pipkin II and two Pipkin I) in six patients were treated by closed reduction of hip dislocation, followed by minimal invasive lag screw osteosynthesis. Cancellous screw(s) were inserted from the incision on the lateral hip through...