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- EISSN 1214-0287 • ISSN 1214-021X
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- published from 2003 – freely available (since 2019 + 2003-2013), published by Elsevier 2014-2018
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Recent achievements
Two our publications got the Award of the Czech Society of Otorhinolaryngology and Head and Neck Surgery for the best publication in 2024
Latest articles
Results 1 to 30 of 61:
Incidence of congenital microcephaly in the Czech Republic: The effect of maternal ageOriginal Research Article
Natálie Friedová, Antonín Šípek, Jan Klaschka, Marek Malý, Pavel Calda, Frantisek Liška, Antonín Šípek, Jr.
J Appl Biomed 23:91-95, 2025 | DOI: 10.32725/jab.2025.009 
Objective: Congenital microcephaly is a diverse group of congenital anomalies characterized by a significantly reduced head circumference at birth. The incidence varies widely across regions. This study focuses on the incidence of microcephaly in the Czech Republic. Methods: A retrospective analysis was conducted using data from the National Registry of Congenital Anomalies of the Czech Republic. All cases coded as microcephaly (Q02 code in the 10th revision of the International Classification of Diseases) between 2000 and 2020 were included. Results: A total of 274 cases of congenital microcephaly were identified, with an incidence rate of 1.22 per...
Unlocking the secrets of aortic pseudoaneurysms - exploring tensile testing of prostheses, anastomoses, and native vessels in the thoracic aorta: A clinical-engineering correlationOriginal Research Article
Sandra Rečičárová, Hynek Chlup, Michael Jonák, Ivan Netuka
J Appl Biomed 23:80-90, 2025 | DOI: 10.32725/jab.2025.008
Introduction: This study examines the mechanical properties of thoracic aortic false aneurysms (TAFA) and how the use of vascular prostheses, native vessels, and anastomoses affects their development. This is done through tensile testing, simulating a Bentall procedure, which is the most common surgery leading to TAFA development. Methods: We conducted uniaxial tensile tests on the native right and left coronary arteries from five cadaveric donors. They were anastomosed to two vascular prostheses in the longitudinal and circumferential directions to assess their mechanical responses under load. Results and discussion: All anastomosis specimens ruptured...
Berberine improves atrial remodeling by regulating the AMPK/PPARα signaling pathway in a rabbit model of atrial fibrillationOriginal Research Article
Yang Wang *, Zhe Sun, Zong-tao Yin, Jian Zhang, Fang-ran Xin, Yin-li Xu, Huai Lan
J Appl Biomed 23:63-79, 2025 | DOI: 10.32725/jab.2025.007
Atrial fibrillation (AF) is a common arrhythmia encountered in clinical practice, characterized by myocardial fibrosis and atrial remodeling as its primary pathological features, and associated with significantly high mortality and disability rates. Currently, there are no specific pharmacological treatments for AF, and traditional anti-arrhythmic drugs have not achieved the desired efficacy, often resulting in a high incidence of adverse drug reactions. Thus, there is an urgent need for the development of novel anti-AF medications. Berberine, the main active component of Coptis chinensis, has been shown to have antiarrhythmic and anti-heart...
The importance of olfactory and trigeminal event-related potentials (OERPs/TERPs) in the assessment of olfactory function in subjects with chronic rhinosinusitis with nasal polyposisOriginal Research Article
Karla Janoušková, Oleksii Vorobiov, Kristýna Mamiňák, David Kalfeřt, Libor Vašina, Petra Dytrych, Nikola Pastorková, Jiří Hložek, Daniel Kovář, Jan Vodička, Václav Masopust, Jaromír Astl, Richard Holý
J Appl Biomed 23:57-62, 2025 | DOI: 10.32725/jab.2025.006
Objectives: Smell impairment (SI) is a well-known symptom of chronic rhinosinusitis with nasal polyps (CRSwNP). The aim of study was to analyze olfactory and trigeminal event-related potentials (OERPs/TERPs) and psychophysical smell tests in subjects with CRSwNP, and its potential role in clinical practice. Methods: Prospective study included 57 subjects. Two investigated groups with CRSwNP and one control group. Group I (n = 20) contained subjects without CRSwNP before septoplasty. Group II (n = 18) contained subjects with CRSwNP without therapy. Group III (n = 19) contained subjects with CRSwNP after intranasal corticosteroid...
Seleno-chitooligosaccharide-induced modulation of intestinal barrier function: Role of inflammatory cytokines, tight junction proteins, and gut microbiota in miceOriginal Research Article
Zhuo-Hong He, Ye Jin, Die Chen, Hui-Xin Zheng, Jia-Er Xiang, Yong-Jun Jiang, Zheng-Shun Wen
J Appl Biomed 23:45-55, 2025 | DOI: 10.32725/jab.2025.003
This study aimed to explore the function of Seleno-chitooligosaccharide (SOA) on the intestinal barrier through regulation of inflammatory cytokines, tight junction protein, and gut microbiota in mice. The results of ELISA assay demonstrated that SOA significantly increased the levels of IL-2, IL-10, and IFN-γ in serum and ileum. Meanwhile, SOA increased the levels of IL-4 in the ileum (p < 0.05). In addition, Diamine Oxidase (DAO) concentration was decreased in ileum by SOA treatments (p < 0.05). The administration of SOA significantly upregulated the expression of ZO-1 and Occludin in the ileum (p < 0.05). By 16S...
Distribution of human leukocyte antigen B27 (HLA-B27) in Slovak patientsOriginal Research Article
Alexandra Pôbišová, Andrea Kollárová, Vladimír Langraf, František Strejček, Ramona Babosová
J Appl Biomed 23:36-44, 2025 | DOI: 10.32725/jab.2025.004
Background and objectives: HLA-B27 is a genetic marker associated with spondyloarthropathies, particularly ankylosing spondylitis and axial spondyloarthritis. While its prevalence varies across populations, no data exist for Slovak patients. This study aimed to determine HLA-B27 prevalence in Slovak patients with suspected spondyloarthropathies and assess differences by sex and age. Methods: A retrospective cohort of 1,614 patients (888 females and 726 males) was analyzed for HLA-B27 status (positive/negative) using reverse hybridisation (HLA-B27 StripAssay). Statistical analyses included Pearson's Chi-square test and non-parametric Mann-Whitney U...
Changes in glucose-related parameters according to LDL-cholesterol concentration ranges in non-diabetic patientsOriginal Research Article
Vladimír Kron, Miroslav Verner, Pavel Smetana, Radka Vrzalová, Eliška Friebergerová, Daniel Martiník, Dagmar Horáková, Ladislav Štěpánek, Lidmila Hamplová, Kvetoslava Kotrbová
J Appl Biomed 23:26-35, 2025 | DOI: 10.32725/jab.2025.005
The study focused on the changes in C-peptide, glycemia, insulin concentration, and insulin resistance according to LDL-cholesterol concentration ranges. The metabolic profile of individuals in the Czech Republic (n = 1840) was classified by quartiles of LDL-cholesterol into four groups with the following ranges: 0.46-2.45 (n = 445), 2.46-3.00 (n = 474), 3.01-3.59 (n = 459), and 3.60-7.18 mmol/l (n = 462). The level of glucose, C-peptide, insulin, and area of parameters during OGTT and HOMA IR were compared with a relevant LDL-cholesterol range. The evaluation involved correlations between LDL-cholesterol and the...
Chemical composition and anticancer activity of Psychotria montana on MCF7 breast cancer cells: insights from in vitro (2D & 3D) studies and in silico analysisOriginal Research Article
Van Hung Hoang, Thi Kieu Oanh Nguyen, Phu Hung Nguyen, Thi Thanh Huong Le, Viet Hoang
J Appl Biomed 23:12-25, 2025 | DOI: 10.32725/jab.2025.002
Aim: This study aimed to investigate the phytochemical composition of Psychotria montana extract (PME) and evaluate its inhibitory effects on MCF7 breast cancer cells. Methods: The chemical composition of PME was analyzed using UPLC-QToF-MS. The effects of PME on cell proliferation were evaluated using the MTT assay. Flow cytometry was used for cell cycle and apoptosis analysis. The effects of PME on the transcription of cell cycle control genes were assessed using real-time PCR. Results: UPLC-QToF-MS analysis revealed major compounds of PME, including terpenoids and flavonoids, with the potential to inhibit proliferation, migration, and induce...
Systematic review of antibacterial potential in calcium oxide and silicon oxide nanoparticles for clinical and environmental infection controlReview Article
Hend Algadi, Mohammed Abdelfatah Alhoot, Laith A. Yaaqoob
J Appl Biomed 23:1-11, 2025 | DOI: 10.32725/jab.2025.001
A substantial threat to worldwide health, the proliferation of antibiotic-resistant bacteria compels researchers to seek innovative antibacterial substances. This systematic review assesses the role of nanoparticles, particularly Calcium oxide and Silicon oxide nanoparticles, in infection control. The article examines the mechanisms by which these nanoparticles act against various bacteria and evaluates their potential as novel agents in infection control strategies. A systematic literature search from 2015 to 2024 encompassing Web of Science, PubMed, Wiley, Science Direct, and Google Scholar, yielded 70 publications meeting the review criteria. This...
Effects of different hemodialysis modalities combined with low-calcium dialysate on mineral metabolism and vascular calcification in maintenance hemodialysis patients with chronic kidney diseaseOriginal Research Article
Jing Wang, Yimian Luo, Xingyu Ji, Hao Xu, Zhenhua Liang, Minjie Zhou
J Appl Biomed 22:228-233, 2024 | DOI: 10.32725/jab.2024.027
Objective: This research investigated the effects of different hemodialysis modalities combined with low-calcium dialysate (LCD) on mineral metabolism and vascular calcification (VC) in maintenance hemodialysis (MHD) patients with chronic kidney disease (CKD). Methods: General data were collected from 192 cases of MHD patients, who were divided into 4 groups according to the randomized numerical table. Each group was given LCD treatment, and conventional hemodialysis (HD), high-flux HD (HFHD), hemodiafiltration (HDF), and HD + hemoperfusion (HP) were performed, respectively. The patients were dialyzed 3 times per week for 4 h each time, and each group...
Endoscopic luminal impedance planimetry of the lower oesophageal sphincter and pylorus in experimental pigs: a pilot studyOriginal Research Article
Jan Bureš, Věra Radochová, Darina Kohoutová, Miroslav Zavoral, Kristina Hugová, Štěpán Suchánek, Ondřej Soukup, Jan Martínek
J Appl Biomed 22:221-227, 2024 | DOI: 10.32725/jab.2024.026
Background/Aims: The functional lumen imaging probe (FLIP) relies on the principle of impedance planimetry that enables direct measurement of intraluminal pressure, cross-sectional areas, and wall biomechanical properties. The aim of our pilot project was to introduce this method to assess function of the lower oesophageal sphincter and pyloric muscle in experimental pigs. Methods: All measurements were accomplished in one session in six adult female pigs (mean weight 34.2 ± 3.6 kg), using the EndoFLIP 1.0 System with EndoFLIP catheters. Five major parameters were evaluated: balloon pressure (mm Hg), estimated diameter (mm), cross-sectional area...
BRAF V600E positive papillary thyroid carcinoma (TERT and TP53 mutation coexistence excluded): Correlation of clinicopathological features and the extent of surgical treatment and its complicationsOriginal Research Article
Jiří Hložek, Jan Rotnágl, Richard Holý, Tereza Hložková, Barbora Peková Bulanová, Vlasta Kuklíková, Běla Bendlová, Jiří Soukup, Petr Hrabal, Jaromír Astl
J Appl Biomed 22:214-220, 2024 | DOI: 10.32725/jab.2024.025
Introduction: Papillary thyroid carcinoma (PTC) frequently harbors the BRAF V600E mutation. Recent research suggests that aggressive behavior in BRAF V600E+ PTC may be due to an undetected mutation in the TERT gene. This study aims to observe the clinicopathological features of BRAF V600+ PTC and correlate them with surgical treatment complications. Methods: A retrospective analysis was conducted on the BRAF V600E+ PTC cohort from July 2019 to January 2023. The histopathological features and surgical treatment (total thyroidectomy - group A, total thyroidectomy + central block neck dissection - group B) complications...
Association between mean platelet volume and adiponectin in patients with metabolic syndromeOriginal Research Article
Milos Marinkovic, Jelena Nesic, Ivica Petrovic, Ivan P Jovanovic, Marija Sekulic, Svetlana Djukic, Aleksandar Djukic
J Appl Biomed 22:208-213, 2024 | DOI: 10.32725/jab.2024.022
Background: Metabolic syndrome is a significant pro-inflammatory and pro-coagulant condition. The clinical association of adiponectin, a mainly antidiabetogenic molecule, and its interaction with platelets and platelet indices remains insufficiently investigated. Objective: The aim of our study was to investigate the association of adiponectin with platelets and platelet indices in patients with metabolic syndrome. Methods: The investigation was conducted as a cross-sectional study involving 113 subjects: 63 patients with the diagnosis of metabolic syndrome, and 50 healthy controls - with clear inclusion and exclusion criteria. The group of patients...
Syringin protects high glucose-induced BMSC injury, cell senescence, and osteoporosis by inhibiting JAK2/STAT3 signalingOriginal Research Article
Yu-Cong Zou, Kai Gao, Bao-Tao Cao, Xiao-Li He, Wei Zheng, Xiao-Fei Wang, Yu-Fu Li, Feng Li, Hua-Jun Wang
J Appl Biomed 22:197-207, 2024 | DOI: 10.32725/jab.2024.021
Background: Acanthopanax senticosus (Rupr. et Maxim.) is commonly used in Traditional Chinese Medicine. Syringin is a major ingredient of phenolic glycoside in Acanthopanax senticosus. Objective: This study was performed to investigate whether Syringin could protect high glucose-induced bone marrow mesenchymal stem cells (BMSCs) injury, cell senescence, and osteoporosis by inhibiting JAK2/STAT3 signaling. Methods: BMSCs isolated from both the tibia and femur of mice were induced for osteogenesis. The cell senescence was induced using the high glucose medium. The cells were treated with 10 and 100 μmol/l Syringin. Immunohistochemistry staining...
Linoleic acid inhibits lipopolysaccharide-induced inflammation by promoting TLR4 regulated autophagy in murine RAW264.7 macrophagesOriginal Research Article
Yin Qin, Kexin Li, Qiuhong Zhang, Jie Liu, Yu Xie, Tingting Zhang, Xiaoliang Wang, Li Zhang, Yu Jiang, Gang Liu
J Appl Biomed 22:185-196, 2024 | DOI: 10.32725/jab.2024.023
Linoleic acid (LA), an essential fatty acid, has emerged as a pivotal regulator in disorders associated with inflammation in recent years; however, the underlying mechanisms are still not completely understood. We utilized network pharmacology and experimental methodologies to elucidate the mechanisms underlying the anti-inflammatory effects of LA. Our network pharmacology analysis revealed that LA shares common targets with sepsis. These targets are enriched in various pathways comprising C-type signaling pathway, PI3K-Akt signaling pathway, toll-like receptor signaling pathway, neutrophil extracellular trap formation, AMPK signaling pathway, and...
The role of chemokines and interleukins in acute lymphoblastic leukemia: a systematic reviewReview Article
Martin Štajer, Jan M. Horáček, Tomáš Kupsa, Pavel Žák
J Appl Biomed 22:165-184, 2024 | DOI: 10.32725/jab.2024.024
Acute lymphoblastic leukemia (ALL) is the most common childhood hematological malignancy, but it also affects adult patients with worse prognosis and outcomes. Leukemic cells benefit from protective mechanisms, which are mediated by intercellular signaling molecules - cytokines. Through these signals, cytokines modulate the biology of leukemic cells and their surroundings, enhancing the proliferation, survival, and chemoresistance of the disease. This ultimately leads to disease progression, refractoriness, and relapse, decreasing the chances of curability and overall survival of the patients. Targeting and modulating these pathological processes without...
In vitro biological activities of Calamintha nepeta L. aqueous extractsOriginal Research Article
Nadia Salhi, Dorien Deluyker, Virginie Bito, Abdelhamid Zaid, Lhoussaine El Rhaffari
J Appl Biomed 22:155-163, 2024 | DOI: 10.32725/jab.2024.017
Aim: This study aimed to investigate the phenolic composition, antioxidant capacity, and toxicity of aqueous extracts of Calamintha nepeta L. leaves and their potential vasorelaxant effects. Methods: Aqueous extracts of Calamintha nepeta L. were prepared by three extraction methods: decoction, infusion, and maceration. The total phenolic contents of the extracts and their antioxidant properties were investigated. The toxicity was evaluated by Artemia salina lethality bioassay. The decoction extract was analyzed by HPLC for its chemical profile and was also used to evaluate the vasorelaxant effect on thoracic aortic rings isolated...
Olfactory event-related potentials (OERPs) and trigeminal event-related potentials (TERPs) in subjects after Covid-19 infection: single-center prospective studyOriginal Research Article
Richard Holý, David Kalfeřt, Libor Vašina, Oleksii Vorobiov, Petra Dytrych, Karla Janoušková, Eva Augste, Shahriar Kashiri, Nikola Pastorková, Kristýna Mamiňák, Jiří Hložek, Daniel Kovář, Jan Vodička, Jaromír Astl
J Appl Biomed 22:149-154, 2024 | DOI: 10.32725/jab.2024.020
Objectives: Olfactory dysfunction (OD) is a common symptom associated with Covid-19. During the Covid-19 pandemic, the importance of psychophysical olfactory tests and electrophysiological olfactory assessment increased. The purpose of the study was to analyze the psychophysical olfactory tests and the post-covid curves of olfactory event-related potentials (OERPs) and trigeminal event-related potentials (TERPs). Methods: The prospective study included 98 subjects (62 females / 36 males). The average age was 42 years (range 21-84 years). Group I (n = 77) contained participants who had been infected with Covid-19. They were enrolled in the study...
Salivary glands - a new site of Helicobacter pylori occurrenceOriginal Research Article
Jan Rotnágl, Jiří Hložek, Richard Holý, Emil Pavlík, David Kalfeřt, Jaromír Astl
J Appl Biomed 22:141-148, 2024 | DOI: 10.32725/jab.2024.018
Objective: The role of Helicobacter pylori (Hp) in the pathological processes of the gastric mucosa is well understood. Decreasing trend in successful eradication of HP from the stomach was observed in last years. This lack of succes is mainly caused by increasing ATB resistance. Nevertheless other possible causes of this phenomenon are being explored. Thus, many studies have focused on the search for extragastric reservoirs as potential sources of persistence or reinfection after successful Hp eradication. The pathological potential of Hp at these localities has also been studied. Methods: Our study aimed to determine the presence of Hp inside...
Anti-NMDAR1 antibody impairs dendritic branching in immature cultured neuronsOriginal Research Article
Pascal Jorratt, Aneta Petrušková
J Appl Biomed 22:136-140, 2024 | DOI: 10.32725/jab.2024.019
Anti-N-methyl D-aspartate receptor (anti-NMDAR) encephalitis is an autoimmune disorder characterized by IgG antibodies targeting NMDAR. The prevalence is remarkably higher in women and some develop the condition during pregnancy. While immunotherapies have shown good outcomes for pregnant mothers and their infants, the impact on early neurodevelopment remains elusive. This study investigates the effects of anti-NMDAR antibody on the development of primary cortical cultures. Anti-NMDAR antibody was administered to the cultures at day in vitro 5 for the following 5 days to assess dendritic branching and arbor complexity, and at day in...
Astragaloside IV confronts amyloid-beta-induced astrocyte senescence via hsp90aa1Original Research Article
Xia Yan, Rongxiang Zeng, Yajun Cao
J Appl Biomed 22:129-135, 2024 | DOI: 10.32725/jab.2024.015
Cell senescence is intensively related to aging and neurodegenerative diseases. This study aimed to explore the effect and targets of Astragaloside IV against amyloid-beta-induced astrocyte senescence. Oligomerized amyloid-beta was prepared to culture with human astrocytes. The effects of Astragaloside IV were assessed based on SA-β-gal staining analysis, senescence markers (p53, p16INK4, and p21WAF1), neurotrophic growth factor levels (qRT-PCR), and cell proliferation (CCK-8 kit). The targets for Astragaloside IV were predicted, and hsp90aa1 protein was verified using molecular docking. After hsp90aa1 overexpression, the effects of Astragaloside...
Today's cancer research and treatment - highly sophisticated and molecularly targeted, yet firmly bolstered in the classical theoriesReview Article
Thomas W. Grunt
J Appl Biomed 22:123-128, 2024 | DOI: 10.32725/jab.2024.016
Cancer research is linked to modern life-sciences, encompassing achievements in virology, yeast-biology, molecular-biology, genetics, systems-biology, bioinformatics, and so on. With these fascinating developments, it's easy to overlook that the fundamental theories and treatment strategies were established in the early 20th century and have remained valid ever since. Therefore, tribute must be paid to the founders of the field. The main hypotheses on carcinogenesis, the genetic model and the metabolic model, and the concept of cancer-treatment with cytotoxic, targeted or metabolic drugs were proposed more than 100 years ago by great minds such as...
RT-qPCR investigation of post-mortem tissues during COVID-19Original Research Article
Zhanna Berdygulova, Elina Maltseva, Yuliya Perfilyeva, Anna Nizkorodova, Andrey Zhigailov, Dinara Naizabayeva, Yekaterina O. Ostapchuk, Saltanat Kuatbekova, Zhaniya Dosmagambet, Moldir Kuatbek, Akerke Bissenbay, Alena Cherusheva, Akzhigit Mashzhan, Nurshat Abdolla, Sanzhar Ashimbekov, Gulnara Ismagulova, Andrey Dmitrovskiy, Seidigapbar Mamadaliyev, Yuriy Skiba
J Appl Biomed 22:115-122, 2024 | DOI: 10.32725/jab.2024.013
In 2020, there were numerous cases in Kazakhstan with clinical symptoms of COVID-19 but negative PCR results in nasopharyngeal and oropharyngeal swabs. The diagnosis was confirmed clinically and by CT scans (computed tomography). The problem with such negative PCR results for SARS-CoV-2 infection confirmation still exists and indicates the need to confirm the diagnosis in the bronchoalveolar lavage in such cases. There is also a lack of information about confirmation of SARS-CoV-2 infection in deceased patients. In this study, various tissue materials, including lungs, bronchi, and trachea, were examined from eight patients who died, presumably from...
Ethyl pyruvate attenuates cellular adhesion and proliferation of diffuse large B-cell lymphoma by targeting c-JunOriginal Research Article
Zhimin Yan, Qiong Zhong, Ling Yan, Wenhong Lai, Xi Xu
J Appl Biomed 22:107-114, 2024 | DOI: 10.32725/jab.2024.014
Diffuse large B-cell lymphoma (DLBCL) stands out as the most common type of malignant cancer, representing the majority of cases of non-Hodgkin's lymphoma. Ethyl pyruvate (EP) is a derivative of pyruvic acid and found to have potent anti-tumor properties. Despite its potential benefits, the impact of EP on DLBCL remains ambiguous. Our objective is to elucidate the role of EP in modulating the development of DLBCL. Analysis of cholecystokinin-8 (CCK-8) revealed that treatment with EP significantly diminished the viability of DLBCL cells. Furthermore, EP administration suppressed colony formation and hindered cell adhesion and invasion in DLBCL cells....
Resveratrol alleviates endoplasmic reticulum stress-induced cell death and improves functional prognosis after traumatic brain injury in miceOriginal Research Article
Qinghua Cao, Lei Gu, Liangzhu Wang, Guangling Sun, Tao Ying, Hang Su, Wei Wang, Zhezhe Sun
J Appl Biomed 22:99-106, 2024 | DOI: 10.32725/jab.2024.008
Resveratrol (RSV) is a polyphenol antioxidant that has been shown to have neuroprotective effects. We sought molecular mechanisms that emphasize the anti-inflammatory activity of RSV in traumatic brain injury (TBI) in mice associated with endoplasmic reticulum stress (ERS). After establishing three experimental groups (sham, TBI, and TBI+RSV), we explored the results of RSV after TBI on ERS and caspase-12 apoptotic pathways. The expression levels of C/EBP homologous protein (CHOP), glucose regulated protein 78kD (GRP78), caspase-3, and caspase-12 in cortical brain tissues were assessed by western blotting. The qPCR analysis was also performed on mRNA...
Possible relationship between respiratory diseases and urinary concentrations of polycyclic aromatic hydrocarbon metabolites - a pilot studyOriginal Research Article
Veronika Gomersall, Kateřina Ciglová, Hana Barošová, Kateřina Hoňková, Ivo Solanský, Anna Pastorková, Radim J. Šrám, Jana Schmuczerová, Jana Pulkrabová
J Appl Biomed 22:89-98, 2024 | DOI: 10.32725/jab.2024.012
This study investigates the potential relationship between exposure to polycyclic aromatic hydrocarbons (PAHs), specifically monohydroxylated metabolites (OH-PAHs), in urine, and the prevalence of respiratory diseases in 2-year-old children residing in two locations within the Czech Republic - České Budějovice (control location) and the historically contaminated mining district of Most. Despite current air quality and lifestyle similarities between the two cities, our research aims to uncover potential long-term health effects, building upon previous data indicating distinctive patterns in the Most population. A total of 248 urine samples were analysed...
The impact of obesity, age, and gender on plasmatic levels of selected glycoprotein biomarkers and miRNA-499 in OSA patientsOriginal Research Article
Tomáš Kostlivý, Martin Pešta, Jindra Windrichová, Petr Škopek, Pavel Klail, Alena Skálová, Břetislav Gál, Radek Kučera, Krista Plicková, Václav Šimánek, David Slouka
J Appl Biomed 22:81-88, 2024 | DOI: 10.32725/jab.2024.011
Background: The current obstructive sleep apnea (OSA) diagnostic uses polysomnography or limited polygraphy and requires specialized personnel and technical equipment. Glycoprotein biomarkers and microRNAs are being explored as a possible new method for screening. We aimed to evaluate whether certain biomarkers and microRNA, previously identified as related to OSA, could be influenced by factors such as gender, age, and obesity level in patients with OSA. Methods: In this retrospective analytical study, patients with moderate to severe OSA (n = 130) were compared with the control group. Serum levels of selected biomarkers and microRNA were taken...
Myo-inositol rescued insulin resistance and dyslipidemia in db/db miceOriginal Research Article
Lingzhi Long, Qi Huang, Tao Song, Zhijie Dai
J Appl Biomed 22:74-80, 2024 | DOI: 10.32725/jab.2024.009
Myo-inositol (MI), present in a variety of foods, is essential in several important processes of cell physiology. In this study, we explored the protective effects of MI against hyperglycemia and dyslipidemia in db/db mice, a typical animal model of type 2 diabetes mellitus (T2DM). MI supplement effectively suppressed the high plasma glucose and insulin levels and markedly relieved the insulin resistance (IR) in the db/db mice, comparable to metformin's effects. In MIN6 pancreatic β cells, MI also restrained the upsurge of insulin secretion stimulated by high-concentration glucose but had no impact on the promoted cell proliferation. Moreover,...
Does vanillic acid affect fracture healing? An experimental study in a rat model of femur fractureOriginal Research Article
Ahmet Yurteri, Numan Mercan, Mehmet Kiliç, Murat Çelik, Fatih Doğar, Ahmet Yildirim
J Appl Biomed 22:67-73, 2024 | DOI: 10.32725/jab.2024.010
Background and objectives: We aimed to determine the effects of vanillic acid (VA) on fracture healing radiologically, histologically, immunohistochemically, and biomechanically using a rat femur open fracture injury model. Methods: 32 male Wistar-Albino rats were used and divided into two groups: the study group (VA) and the control group. From the time they were operated on until they were sacrificed, the rats in the study group were given 100 mg/kg/day VA by oral gavage. After sacrification, the femurs were analyzed. Results: It was observed that the Huo histological scoring was significantly higher in the VA group (p = 0.001), and the ratio...
Rapid triage and transfer system for patients with proven Covid-19 at emergency departmentOriginal Research Article
Martin Jakl, Jana Berková, Tomáš Veleta, Vladimír Palička, Petra Polcarová, Jan Smetana, Petr Grenar, Martina Čermáková, Jan Vaněk, Jan M. Horáček, Jaromír Kočí
J Appl Biomed 22:59-65, 2024 | DOI: 10.32725/jab.2024.006
Background: COVID-19 is a viral disease notorious for frequent worldwide outbreaks. It is difficult to control, thereby resulting in overload of the healthcare system. A possible solution to prevent overcrowding is rapid triage of patients, which makes it possible to focus care on the high-risk patients and minimize the impact of crowding on patient prognosis. Methods: The triage algorithm assessed self-sufficiency, oximetry, systolic blood pressure, and the Glasgow coma scale. Compliance with the triage protocol was defined as fulfillment of all protocol steps, including assignment of the correct level of care. Triage was considered successful if...