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- 2.0 – 2-year Impact Factor
- 1.8 – 5-year Impact Factor
- EISSN 1214-0287 • ISSN 1214-021X
- double-blind evaluation of manuscript by independent international experts
- fast on-line publication of accepted manuscripts
- open access
- published from 2003 – freely available (since 2019 + 2003-2013), published by Elsevier 2014-2018
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Recent achievements
Two our publications got the Award of the Czech Society of Otorhinolaryngology and Head and Neck Surgery for the best publication in 2024
Latest articles
Results 31 to 46 of 46:
Astragaloside IV confronts amyloid-beta-induced astrocyte senescence via hsp90aa1Original Research Article
Xia Yan, Rongxiang Zeng, Yajun Cao
J Appl Biomed 22:129-135, 2024 | DOI: 10.32725/jab.2024.015 
Cell senescence is intensively related to aging and neurodegenerative diseases. This study aimed to explore the effect and targets of Astragaloside IV against amyloid-beta-induced astrocyte senescence. Oligomerized amyloid-beta was prepared to culture with human astrocytes. The effects of Astragaloside IV were assessed based on SA-β-gal staining analysis, senescence markers (p53, p16INK4, and p21WAF1), neurotrophic growth factor levels (qRT-PCR), and cell proliferation (CCK-8 kit). The targets for Astragaloside IV were predicted, and hsp90aa1 protein was verified using molecular docking. After hsp90aa1 overexpression, the effects of Astragaloside...
Today's cancer research and treatment - highly sophisticated and molecularly targeted, yet firmly bolstered in the classical theoriesReview Article
Thomas W. Grunt
J Appl Biomed 22:123-128, 2024 | DOI: 10.32725/jab.2024.016
Cancer research is linked to modern life-sciences, encompassing achievements in virology, yeast-biology, molecular-biology, genetics, systems-biology, bioinformatics, and so on. With these fascinating developments, it's easy to overlook that the fundamental theories and treatment strategies were established in the early 20th century and have remained valid ever since. Therefore, tribute must be paid to the founders of the field. The main hypotheses on carcinogenesis, the genetic model and the metabolic model, and the concept of cancer-treatment with cytotoxic, targeted or metabolic drugs were proposed more than 100 years ago by great minds such as...
RT-qPCR investigation of post-mortem tissues during COVID-19Original Research Article
Zhanna Berdygulova, Elina Maltseva, Yuliya Perfilyeva, Anna Nizkorodova, Andrey Zhigailov, Dinara Naizabayeva, Yekaterina O. Ostapchuk, Saltanat Kuatbekova, Zhaniya Dosmagambet, Moldir Kuatbek, Akerke Bissenbay, Alena Cherusheva, Akzhigit Mashzhan, Nurshat Abdolla, Sanzhar Ashimbekov, Gulnara Ismagulova, Andrey Dmitrovskiy, Seidigapbar Mamadaliyev, Yuriy Skiba
J Appl Biomed 22:115-122, 2024 | DOI: 10.32725/jab.2024.013
In 2020, there were numerous cases in Kazakhstan with clinical symptoms of COVID-19 but negative PCR results in nasopharyngeal and oropharyngeal swabs. The diagnosis was confirmed clinically and by CT scans (computed tomography). The problem with such negative PCR results for SARS-CoV-2 infection confirmation still exists and indicates the need to confirm the diagnosis in the bronchoalveolar lavage in such cases. There is also a lack of information about confirmation of SARS-CoV-2 infection in deceased patients. In this study, various tissue materials, including lungs, bronchi, and trachea, were examined from eight patients who died, presumably from...
Ethyl pyruvate attenuates cellular adhesion and proliferation of diffuse large B-cell lymphoma by targeting c-JunOriginal Research Article
Zhimin Yan, Qiong Zhong, Ling Yan, Wenhong Lai, Xi Xu
J Appl Biomed 22:107-114, 2024 | DOI: 10.32725/jab.2024.014
Diffuse large B-cell lymphoma (DLBCL) stands out as the most common type of malignant cancer, representing the majority of cases of non-Hodgkin's lymphoma. Ethyl pyruvate (EP) is a derivative of pyruvic acid and found to have potent anti-tumor properties. Despite its potential benefits, the impact of EP on DLBCL remains ambiguous. Our objective is to elucidate the role of EP in modulating the development of DLBCL. Analysis of cholecystokinin-8 (CCK-8) revealed that treatment with EP significantly diminished the viability of DLBCL cells. Furthermore, EP administration suppressed colony formation and hindered cell adhesion and invasion in DLBCL cells....
Resveratrol alleviates endoplasmic reticulum stress-induced cell death and improves functional prognosis after traumatic brain injury in miceOriginal Research Article
Qinghua Cao, Lei Gu, Liangzhu Wang, Guangling Sun, Tao Ying, Hang Su, Wei Wang, Zhezhe Sun
J Appl Biomed 22:99-106, 2024 | DOI: 10.32725/jab.2024.008
Resveratrol (RSV) is a polyphenol antioxidant that has been shown to have neuroprotective effects. We sought molecular mechanisms that emphasize the anti-inflammatory activity of RSV in traumatic brain injury (TBI) in mice associated with endoplasmic reticulum stress (ERS). After establishing three experimental groups (sham, TBI, and TBI+RSV), we explored the results of RSV after TBI on ERS and caspase-12 apoptotic pathways. The expression levels of C/EBP homologous protein (CHOP), glucose regulated protein 78kD (GRP78), caspase-3, and caspase-12 in cortical brain tissues were assessed by western blotting. The qPCR analysis was also performed on mRNA...
Possible relationship between respiratory diseases and urinary concentrations of polycyclic aromatic hydrocarbon metabolites - a pilot studyOriginal Research Article
Veronika Gomersall, Kateřina Ciglová, Hana Barošová, Kateřina Hoňková, Ivo Solanský, Anna Pastorková, Radim J. Šrám, Jana Schmuczerová, Jana Pulkrabová
J Appl Biomed 22:89-98, 2024 | DOI: 10.32725/jab.2024.012
This study investigates the potential relationship between exposure to polycyclic aromatic hydrocarbons (PAHs), specifically monohydroxylated metabolites (OH-PAHs), in urine, and the prevalence of respiratory diseases in 2-year-old children residing in two locations within the Czech Republic - České Budějovice (control location) and the historically contaminated mining district of Most. Despite current air quality and lifestyle similarities between the two cities, our research aims to uncover potential long-term health effects, building upon previous data indicating distinctive patterns in the Most population. A total of 248 urine samples were analysed...
The impact of obesity, age, and gender on plasmatic levels of selected glycoprotein biomarkers and miRNA-499 in OSA patientsOriginal Research Article
Tomáš Kostlivý, Martin Pešta, Jindra Windrichová, Petr Škopek, Pavel Klail, Alena Skálová, Břetislav Gál, Radek Kučera, Krista Plicková, Václav Šimánek, David Slouka
J Appl Biomed 22:81-88, 2024 | DOI: 10.32725/jab.2024.011
Background: The current obstructive sleep apnea (OSA) diagnostic uses polysomnography or limited polygraphy and requires specialized personnel and technical equipment. Glycoprotein biomarkers and microRNAs are being explored as a possible new method for screening. We aimed to evaluate whether certain biomarkers and microRNA, previously identified as related to OSA, could be influenced by factors such as gender, age, and obesity level in patients with OSA. Methods: In this retrospective analytical study, patients with moderate to severe OSA (n = 130) were compared with the control group. Serum levels of selected biomarkers and microRNA were taken...
Myo-inositol rescued insulin resistance and dyslipidemia in db/db miceOriginal Research Article
Lingzhi Long, Qi Huang, Tao Song, Zhijie Dai
J Appl Biomed 22:74-80, 2024 | DOI: 10.32725/jab.2024.009
Myo-inositol (MI), present in a variety of foods, is essential in several important processes of cell physiology. In this study, we explored the protective effects of MI against hyperglycemia and dyslipidemia in db/db mice, a typical animal model of type 2 diabetes mellitus (T2DM). MI supplement effectively suppressed the high plasma glucose and insulin levels and markedly relieved the insulin resistance (IR) in the db/db mice, comparable to metformin's effects. In MIN6 pancreatic β cells, MI also restrained the upsurge of insulin secretion stimulated by high-concentration glucose but had no impact on the promoted cell proliferation. Moreover,...
Does vanillic acid affect fracture healing? An experimental study in a rat model of femur fractureOriginal Research Article
Ahmet Yurteri, Numan Mercan, Mehmet Kiliç, Murat Çelik, Fatih Doğar, Ahmet Yildirim
J Appl Biomed 22:67-73, 2024 | DOI: 10.32725/jab.2024.010
Background and objectives: We aimed to determine the effects of vanillic acid (VA) on fracture healing radiologically, histologically, immunohistochemically, and biomechanically using a rat femur open fracture injury model. Methods: 32 male Wistar-Albino rats were used and divided into two groups: the study group (VA) and the control group. From the time they were operated on until they were sacrificed, the rats in the study group were given 100 mg/kg/day VA by oral gavage. After sacrification, the femurs were analyzed. Results: It was observed that the Huo histological scoring was significantly higher in the VA group (p = 0.001), and the ratio...
Rapid triage and transfer system for patients with proven Covid-19 at emergency departmentOriginal Research Article
Martin Jakl, Jana Berková, Tomáš Veleta, Vladimír Palička, Petra Polcarová, Jan Smetana, Petr Grenar, Martina Čermáková, Jan Vaněk, Jan M. Horáček, Jaromír Kočí
J Appl Biomed 22:59-65, 2024 | DOI: 10.32725/jab.2024.006
Background: COVID-19 is a viral disease notorious for frequent worldwide outbreaks. It is difficult to control, thereby resulting in overload of the healthcare system. A possible solution to prevent overcrowding is rapid triage of patients, which makes it possible to focus care on the high-risk patients and minimize the impact of crowding on patient prognosis. Methods: The triage algorithm assessed self-sufficiency, oximetry, systolic blood pressure, and the Glasgow coma scale. Compliance with the triage protocol was defined as fulfillment of all protocol steps, including assignment of the correct level of care. Triage was considered successful if...
Amelioration of obesity induction by a high-fat diet and related inflammation by Phasa fish (Setipinna phasa) oil in BALB/c miceOriginal Research Article
Titli Panchali, Ananya Dutta, Pipika Das, Amina Khatun, Riya Kar, Subhadeep Mondal, Keshab Chandra Mondal, Sudipta Chakrabarti, Kuntal Ghosh, Shrabani Pradhan
J Appl Biomed 22:49-58, 2024 | DOI: 10.32725/jab.2024.003
We have extracted and characterized Phasa fish (Setipinna phasa) oil for the first time to evaluate the anti-obesity and related anti-inflammatory effects on obese mice. Inbred male albino BALB/c mice were segregated into three categories: control (C), Obese control group (OC), and Phasa fish oil treated group (TX). To establish the potentiality of Setipinna phasa oil for its anti-obesity and anti-inflammatory properties, it was extracted and characterized using GC-MS method. To evaluate the anti-obesity effect, different parameters were considered, such as body weight, lipid composition, obesity, and obesity associated inflammation....
Endoplasmic reticulum stress-related protein GRP78 and CHOP levels in synovial fluid correlate with disease progression of primary knee osteoarthritis: A cross-sectional studyOriginal Research Article
Dan-Dan Liu, Yuan-Chuang Zhao, Hai-Hong Li, Lian-Jun Yin, Jun-Qi Chen, Gang Liu
J Appl Biomed 22:40-48, 2024 | DOI: 10.32725/jab.2024.001
Background: Endoplasmic reticulum (ER) stress has been shown to play an important role in osteoarthritis (OA). Objective: This study was aimed at assessing the relationship of endoplasmic reticulum (ER) stress-related glucose-regulated protein 78 (GRP78) and CCAAT/enhancer-binding protein homologous protein (CHOP) concentrations in the serum/synovial fluid (SF) with disease severity of primary knee osteoarthritis (pkOA). Methods: Patients with pkOA together with healthy individuals were consecutively recruited from our hospital. The levels of GRP78 and CHOP in serum / SF were detected using enzyme-linked immunosorbent assay. The levels of IL-6 and...
Anti-inflammatory effect of luteoloside against methylglyoxal induced human dental pulp cellsOriginal Research Article
Ji-Eun Kim, Pradhan Paras Man, Sungil Jang, Ho-Keun Yi *
J Appl Biomed 22:33-39, 2024 | DOI: 10.32725/jab.2024.002
Purpose: The aim of this study was to investigate whether luteoloside, a flavonoid, could protect human dental pulp cells (HDPCs) against inflammation and oxidative stress induced by methylglyoxal (MGO), one of the advanced glycated end products (AGE) substances. Methods: HDPCs were stimulated with MGO and treated with luteoloside. MTT assay was used to determine cell viability. Protein expression was measured via western blotting. Reactive oxygen species (ROS) were measured with a Muse Cell Analyzer. Alkaline phosphatase activity (ALP) and Alizarin red staining were used for mineralization assay. Results: Luteoloside down-regulated the expression...
Dictyophora indusiata polysaccharide mediates priming of the NLRP3 inflammasome activation via TLR4/ NF-κB signaling pathway to exert immunostimulatory effectsOriginal Research Article
Youyi Liu, Huanxiao Zhang, Yuxuan Li, Hanqian Zha, Yujie Gao, Hui Chen, Yalin Wang, Tongxin Zhou, Chao Deng
J Appl Biomed 22:23-32, 2024 | DOI: 10.32725/jab.2024.005
Dictyophora indusiata, commonly known as bamboo fungus, is a type of edible mushroom that is highly popular worldwide for its rich flavor and nutritional value. It is also recognized for its pharmaceutical efficacy, with medicinal benefits attributed to its consumption. One of the most important components of Dictyophora indusiata is polysaccharide, which has been acknowledged as a promising regulator of biological response due to its immunostimulatory and anti-inflammatory properties. However, the specific roles of polysaccharide in modulating the NOD-like receptor protein 3 (NLRP3) inflammasome activation within macrophages remain relatively...
Prenatal exposure to polycyclic aromatic hydrocarbons and growth parametersOriginal Research Article
Radim J. Šrám, Ivo Solanský, Anna Pastorková, Miloš Velemínský, Jr., Miloš Velemínský, Kateřina Hoňková, Hana Barošová, Jana Schmuczerová, Kateřina Urbancová, Darina Dvořáková, Jana Pulkrabová
J Appl Biomed 22:12-22, 2024 | DOI: 10.32725/jab.2024.004
Background and objectives: It has long been known that airborne polycyclic aromatic hydrocarbons (PAHs) can negatively affect pregnancy and birth outcomes, such as birth weight, fetal development, and placental growth factors. However, similar studies yield divergent results. Our goal was to estimate the amount of monohydroxylated PAH (OH-PAH) metabolites in the urine of pregnant women/mothers and their newborns in relation to birth outcomes, such as placenta weight, Apgar 5', and the growth parameters of children up to the age of two. Methods: Two cohorts of children born in 2013 and 2014 during the summer and winter seasons in the Czech Republic...
Selected widely prescribed pharmaceuticals: toxicity of the drugs and the products of their photochemical degradation to aquatic organismsReview Article
Šárka Klementová *, Martina Poncarová
J Appl Biomed 22:1-11, 2024 | DOI: 10.32725/jab.2024.007
Cholesterol-lowering drugs, antidiabetics, antiarrhythmics, antidepressants, and antibiotics belong to the most prescribed drugs worldwide. Because of the manufacture, excretion, and improper disposal of leftover drugs, the drugs enter waste waters and, subsequently, surface waters. They have been detected in surface waters all over the world, from concentrations of ng/l to concentrations several orders of magnitude higher. Since pharmaceuticals are designed to be both biologically and chemically stable, photochemical degradation by sun radiation represents a way of transformation in the natural environment. This review provides a survey of how selected...