Journal of Applied Biomedicine, 2006 (vol. 4), issue 2
Original Research Article
Genetic determination of an endothelial function and the size of the heart sections in juvenile hypertensives
Věra Adámková, Jaroslav A. Hubáček, Helena Pistulková, Hana Malínská, Jelena Skibová
J Appl Biomed 4:59-65, 2006 | DOI: 10.32725/jab.2006.005 
Arterial hypertension is a polygenic disease and about 50 candidate genes have been analysed. We followed the size of the heart sections and the endothelial function in juvenile hypertensives according to the polymorphisms in the genes for angiotensin converting enzyme (I/D), endothelin 1 (Lys198/Asn) and endothelin 1 converting enzyme (Thr341/Ile).We observed 44 juvenile hypertensives and 94 controls of the same age. An echocardiographic examination was carried out during standard examinations. The endothelial function was analysed with the use of ultrasound with high acuity after revoking a reactive hyperaemia. The genotypisations were performed...
Reactivation study of pyridinium oximes for acetylcholinesterases inhibited by paraoxon or DFP
Tae-Hyuk Kim, Kyung-Ae Oh, No-Joong Park, No-Sang Park, Yeong Joon Kim, Eul Kyun Yum, Young-Sik Jung
J Appl Biomed 4:67-72, 2006 | DOI: 10.32725/jab.2006.006
We tested the potency to reactivate AChE inhibited by diisopropyl fluorophosphates (DFP) by using bis-pyridinium oxime reactivators connected with CH2CH2OCH2CH2 linkers between two pyridinium rings. The potency was strongly dependent on oxime functional groups, and the bis-oxime derivatives 1,1-[Oxybis(ethylene)]-bis[4-(hydroxyimino)methyl]pyridinium dibromide (1) and 1,1-[Oxybis(ethylene)]-bis[2-(hydroxyimino)methyl]pyridinium dichloride (2) are more potent than mono-oxime compounds 1-(4-hydroxyiminomethyl-1-pyridino)-5-(4-carbamoyl-1-pyridino)-3-oxapentane dibromide (3) and 1-(3-hydroxyiminomethyl-1-pyridino)-5-(4-carbamoyl-1-pyridino)-3-oxapentane...
Chronobiology's progress. Part II, chronomics for an immediately applicable biomedicine
Franz Halberg, Germaine Cornélissen, George Katinas, Levan Tvildiani, Marina Gigolashvili, Ketevan Janashia, Tim Toba, Miguel Revilla, Philip Regal, Robert B. Sothern, Hans W. Wendt, Zhengrong Wang, Michal Zeman, Rita Jozsa, R.B. Singh, Gen Mitsutake, Sergei M. Chibisov, Jong Lee, Dan Holley, James E. Holte, Robert P. Sonkowsky, Othild Schwartzkopff, Patrick Delmore, Kuniaki Otsuka, Earl E. Bakken, Jerzy Czaplicki, the International BIOCOS Group
J Appl Biomed 4:73-86, 2006 | DOI: 10.32725/jab.2006.007
Chronomic cardiovascular surveillance serves to recognise and treat any risk elevation as well as overt disease, and to ascertain whether treatment is effective and, if so, for how long treatment effects lasts, be it for lowering an increased risk and/or in surveilling the success or failure of treatment. A treatment-associated increase in circadian amplitude of blood pressure (BP) may induce iatrogenic overswinging, also dubbed CHAT ( circadian hyper- amplitude- tension), in some patients, thereby increasing cardiovascular disease risk unknowingly to care provider and receiver.
GOSA, a simulated annealing-based program for global optimization of nonlinear problems, also reveals transyears
Jerzy Czaplicki, Germaine Cornélissen, Franz Halberg
J Appl Biomed 4:87-94, 2006 | DOI: 10.32725/jab.2006.008
Transyears in biology have been documented thus far by the extended cosinor approach, including linear-nonlinear rhythmometry. We here confirm the existence of transyears by simulated annealing, a method originally developed for a much broader use, but described and introduced herein for validating its application to time series. The method is illustrated both on an artificial test case with known components and on biological data. We provide a table comparing results by the two methods and trust that the procedure will serve the budding sciences of chronobiology (the study of mechanisms underlying biological time structure), chronomics (the mapping...
Imatinib mesylate affects tyrosine kinase activity in both leukemic and normal primary mononuclear blood cells
Kateřina Kuželová, Dana Grebeňová, Michaela Pluskalová, Iuri Marinov, Hana Klamová, Zbyněk Hrkal
J Appl Biomed 4:95-104, 2006 | DOI: 10.32725/jab.2006.009
Tyrosine kinase (TK) activity in primary mononuclear blood cells (MNBC) derived from chronic myelogenous leukemia (CML) patients in the chronic phase as well as from healthy donors was measured by a sensitive time-resolved fluorescence method using the Delfia® Tyrosine Kinase kit. The level of phosphotyrosine was assessed in parallel by flow-cytometry. The experimental protocol for Delfia® was optimized using a K562 cell line. A large part (20 to 50%) of the fluorescence signal from K562 cells was sensitive to Imatinib mesylate, an inhibitor of Bcr-Abl tyrosine kinase, which is currently the leading drug in CML treatment. In primary MNBC, the...
Freshwater microcrustacean Daphnia magna Straus as an early screen model to compare toxicity of acetylcholinesterase inhibitors
Šárka Veselá, Vlastimil Ondruška, Kamil Kuča, Jiří Patočka
J Appl Biomed 4:105-110, 2006 | DOI: 10.32725/jab.2006.010
Daphnia magna is a freshwater microcrustacean which is often used for acute and chronic toxicity testing in aquatic ecotoxicology. Recently, tests with daphnids have been used to prescreen the toxicity of newly synthesized acetylcholinesterase reactivators (oximes), which appear as weak inhibitors of acetylcholinesterase (AChE). In our study we investigated and compared the toxicity of five reversible acetylcholinesterase inhibitors, two of them non ionic (tacrine and 7-MEOTA) and three ionic (berberine chloride, 1-methyltacridium iodide and ethidium bromide). Tests were carried out according to the methodology described in the standard EN ISO...