Journal of Applied Biomedicine, 2009 (vol. 7), issue 2
Original Research Article
Is a high dose of Huperzine A really suitable for pretreatment against high doses of soman?
Jana ®ďárová Karasová, Jiří Bajgar, Ladislav Novotný, Kamil Kuča
J Appl Biomed 7:93-99, 2009 | DOI: 10.32725/jab.2009.009 
Huperzine A (Hup A) is a reversible AChE inhibitor that crosses the blood-brain barrier. It is presently approved for, or is in a course of clinical trials for, the treatment of Alzheimer's disease. This compound has also been successfully tested for the pretreatment of organophosphate poisoning. Organophosphate nerve agents are potent irreversible inhibitors of acetylcholinesterase in the central and also in the peripheral compartment. In this study Hup A in a higher dose (500 μg/kg) was tested as a prophylaxis against a high, mainly centrally acting, nerve agent (soman). According to the results obtained, Hup A in this dosage was not able to protect...
Glutathione, glutathione-dependent enzymes and antioxidant status in gastric carcinoma patients
Palanisamy Pasupathi, Ganesan Saravanan, Palanisamy Chinnaswamy, Govindaswamy Bakthavathsalam
J Appl Biomed 7:101-109, 2009 | DOI: 10.32725/jab.2009.010
Oxidative stress plays an important role in malignant transformation and is postulated to be associated with increased lipid peroxidation. The aim of this study was to determine the extent of lipid peroxidation with the antioxidant status in patients with gastric cancer. The study population consisted of fifty newly diagnosed gastric cancer patients and an equal number of age- and sex-matched healthy control subjects. Lipid peroxidation as evidenced by thiobarbutric acid reactive substances (TBARS), and also the status of enzymatic (superoxide dismutase - SOD, catalase - CAT, glutathione peroxidase - GPx, glutathione reductase - GR, glutathione S-transferase...
The translocation of AgNORs in large nucleoli of early granulocyte progenitors in patients suffering from chronic phase of chronic myeloid leukaemia
Karel Smetana, Ilona Jirásková, Dana Mikulenková, Hana Klamová
J Appl Biomed 7:111-114, 2009 | DOI: 10.32725/jab.2009.011
The present study was undertaken to provide more information of the translocation of AgNORs to the nucleolar periphery in human early granulocyte progenitors such as myeloblasts and promyelocytes. The bone marrow smears of patients untreated or treated with the cytostatic therapy appeared to be a convenient model for such study because they possessed a satisfactory number of these cells. The translocation of AgNORs to the nucleolar periphery in early granulocytic progenitors was observed in all studied patients but with different incidence. Since the translocation of AgNORs to the nucleolar periphery was induced in experimental studies in vitro...
Reviews
Recent advances in the research on biological roles of dietary polyamines in man
Pavel Kalač
J Appl Biomed 7:65-74, 2009 | DOI: 10.32725/jab.2009.007
The ubiquitous polyamines putrescine, spermidine and spermine fulfil an array of physiological roles in man. In particular, their participation in cell growth and proliferation has been of great interest in relation to their roles in tumour growth and in wound healing. Both endogenous and dietary polyamines take part in such processes. The deprivation of exogenous polyamines emerges as a promising strategy in tumour therapy. Thus, reliable information on their content in foods is needed for dieticians. This review continues our previous comprehensive review on the topic, summarising data on the polyamine content in foods published from 2005 to April...
Gold and nano-gold in medicine: overview, toxicology and perspectives
Nagender Reddy Panyala, Eladia María Peña-Méndez, Josef Havel
J Appl Biomed 7:75-91, 2009 | DOI: 10.32725/jab.2009.008
Nano-technology has entered the field of medicine in recent decades and many of the nanomaterials developed have already had a high impact on health care. Among nanomaterials, gold nanoparticles (GNPs) and gold quantum dots (QDs) are receiving significant attention because their unique physical, chemical and biological properties are quite different from the bulk of their counterparts. In this article, after a brief historical overview, the use of gold and nano-gold in medicine is reviewed, analyzed, and discussed. The review particularly deals with the use of GNPs and bio-conjugated GNPs in cancer treatment, drug or gene delivery, DNA detection, biomedical...