Journal of Applied Biomedicine, 2019 (vol. 17), issue 4
Original Research Article
The frequency and consequences of multipolar mitoses in undifferentiated embryonic stem cells
Veronika Pospíšilová, Milan Ešner, Iveta Červenková, Radek Fedr, Jean-Yvez Tinevez, Aleš Hampl, Martin Anger
J Appl Biomed 17:209-217, 2019 | DOI: 10.32725/jab.2019.018 
Embryonic stem (ES) cells are pluripotent cells widely used in cell therapy and tissue engineering. However, the broader clinical applications of ES cells are limited by their genomic instability and karyotypic abnormalities. Thus, understanding the mechanisms underlying ES cell karyotypic abnormalities is critical to optimizing their clinical use. In this study, we focused on proliferating human and mouse ES cells undergoing multipolar divisions. Specifically, we analyzed the frequency and outcomes of such divisions using a combination of time-lapse microscopy and cell tracking. This revealed that cells resulting from multipolar divisions were not...
Chokeberry juice supplementation in type 2 diabetic patients - impact on health status
Milica Milutinović, Radmila Veličković Radovanović, Katarina Šavikin, Saša Radenković, Marjan Arvandi, Milica Pešić, Milica Kostić, Bojana Miladinović, Suzana Branković, Dušanka Kitić
J Appl Biomed 17:218-224, 2019 | DOI: 10.32725/jab.2019.020
Recent literature evidence indicates the potential use of chokeberry preparations in the prevention and treatment of some chronic noncommunicable diseases. The aim of the present study was to evaluate the effects of the three months oral chokeberry juice supplementation in type 2 diabetic patients, as well as its influence on hematological parameters and certain parameters of the renal dysfunction. The study was designed as an open-label trial, which included 35 patients who have received the herbal supplement, polyphenol-rich chokeberry juice (150 ml/day, three times a day for 50 ml), in addition to their standard therapy. Chokeberry juice as a rich...
The evaluation of oxidative damage of DNA after poisoning with nerve agents
Jiri Kassa *, Rudolf Stetina
J Appl Biomed 17:225-230, 2019 | DOI: 10.32725/jab.2019.017
The potency of three nerve agents (sarin, soman, tabun) to induce oxidative damage of DNA in lymphocytes, liver and brain during lethal or sublethal poisoning was investigated. The single strand breaks or oxidative base DNA damage was evaluated with the help of Comet assay and a specific enzyme able to detect oxidative bases of DNA (endonuclease III). While sarin and soman administered at sublethal doses corresponding to 50% of their LD50 values were not able to induce oxidative damage of DNA, their lethal dose (LD50) induced the significant increase of the number of oxidative bases in DNA of hepatocytes. In addition, tabun administered at lethal dose...
Review Article
Image-guided left ventricular lead placement in cardiac resynchronization therapy: focused on image fusion methods
Premysl Hajek, Iva Safarikova, Jan Baxa
J Appl Biomed 17:199-208, 2019 | DOI: 10.32725/jab.2019.019
Cardiac resynchronization therapy is an effective and widely accessible treatment for patients with advanced, drug-refractory heart failure. It has been shown to reverse maladaptive ventricular remodeling, increase exercise capacity, and lower hospitalization and mortality rates. However, there still exists a considerable proportion of patients who do not respond favorably to the therapy. Tailored left ventricular (LV) lead positioning instead of empiric implantation is thought to have the greatest potential to increase response rates. In our paper, we focus on the rationale for guided LV lead implantation and provide a review of the non-invasive imaging...