Journal of Applied Biomedicine, 2020 (vol. 18), issue 4
Original Research Article
Automatic multi-class intertrochanteric femur fracture detection from CT images based on AO/OTA classification using faster R-CNN-BO method
Sun-Jung Yoon, Tae Hyong Kim, Su-Bin Joo, Seung Eel Oh
J Appl Biomed 18:97-105, 2020 | DOI: 10.32725/jab.2020.013 
Intertrochanteric (IT) femur fractures are the most common fractures in elderly people, and they lead to significant morbidity, mortality, and reduced quality of life. The different types of fractures require a careful definition to ensure accurate surgical planning and reduce the operation time, healing time, and number of surgical failures. In this study, a deep learning-based automatic multi-class IT fracture detection model was developed using computed tomography (CT) images and based on the AO/OTA classification method. The original CT image was resized and rearranged according to the fracture location and an unsharp masking filter was applied....
Action of carvacrol in Salmonella Typhimurium biofilm: A proteomic study
Daliah Alves Coelho Trevisan, Paula Aline Zanetti Campanerut-Sá, Alex Fiori da Silva, Andreia Farias Pereira Batista, Flavio Augusto Vicente Seixas, Rosane Marina Peralta, Anacharis Babeto de Sá-Nakanishi, Benício Alves de Abreu Filho, Miguel Machinski Junior, Jane Martha Graton Mikcha
J Appl Biomed 18:106-114, 2020 | DOI: 10.32725/jab.2020.014
Carvacrol presents action in Salmonella Typhimurium biofilms, however the antibiofilm mechanism of this compound has not been fully established yet. In the present study, the aim was to evaluate protein profile changes in S. Typhimurium biofilm treated with carvacrol. Proteomic analysis of treated versus untreated biofilm showed several changes in proteins involved with S. Typhimurium biofilm and antioxidant activity. The proteins DsbA (thiol: disulfide interchange protein DsbA), LuxS (S-ribosylhomocysteine lyase), DksA (RNA polymerase binding transcription factor DksA), and SODs (superoxide dismutases) A, B and C had their synthesis...
Rosuvastatin, Perindopril and Ezetimibe loaded instant release buccal films: Development and in vitro characterization
Sobia Anwar, Muhammad Zaman, Maria Abdul Ghafoor Raja, Asif Mahmood, Muhammad Wahab Amjad
J Appl Biomed 18:115-125, 2020 | DOI: 10.32725/jab.2020.015
Background: Rosuvastatin Calcium and Ezetimibe are used to control cholesterol level while Perindopril Erbumine is used to treat hypertension. Hepatic metabolism reduces the therapeutic effect of these drugs. Objective: Instant release buccal films (IRBFs) could possibly be a solution to this issue. The objective of the study was to formulate IRBFs of Rosuvastatin Calcium, Perindopril Erbumine and Ezetimibe using solvent casting technique. Methods: Polymers used to prepare IRBFs included hydroxypropyl methylcellulose (HPMC E5), PEG 400 (as plasticizer) and Tween 80 (as surfactant). Solvent casting technique was used to fabricate the films, followed...
The effects of Rosmarinus officinalis L. essential oil and its nanoemulsion on dyslipidemic Wistar rats
Ana Paula Santos Rodrigues, Belmira Silva Faria e Souza, Albenise Santana Alves Barros, Helison de Oliveira Carvalho, Jonatas Lobato Duarte, Letícia Elizandra Mehl Boettger, Robson Barbosa, Adriana Maciel Ferreira, Irlon Maciel Ferreira, Caio Pinho Fernandes, Arlindo César Matias Pereira, José Carlos Tavares Carvalho
J Appl Biomed 18:126-135, 2020 | DOI: 10.32725/jab.2020.016
Dyslipidemias are lipid metabolism alterations that cause increased levels of serum lipoprotein, cholesterol, and triglycerides. These alterations are associated with a higher incidence of cardiovascular diseases and are a risk factor for atherosclerosis development. This study aimed to evaluate the effect of Rosmarinus officinalis essential oil (EORO, 100 mg/kg) and its nanoemulsion (NEORO, 500 µg/kg) on Triton and coconut saturated-fat-induced (CSF) dyslipidemias using Wistar rats. The phytochemical evaluation of EORO performed by gas chromatography-mass spectroscopy (GC-MS) revealed 1,8-cineole (33.70%), camphor (27.68%), limonene (21.99%),...
Alterations of glycaemia, insulin resistance and body mass index within the C-peptide optimal range in non-diabetic patients
Vladimír Kron, Miroslav Verner, Pavel Smetana, Jana Janoutová, Vladimír Janout, Karel Martiník
J Appl Biomed 18:136-142, 2020 | DOI: 10.32725/jab.2020.018
The study focused on changes or cut-offs of glycaemia, insulin resistance and body mass index within the C-peptide reference range (260-1730 pmol/l). The metabolic profile of individuals in the Czech Republic without diabetes (n = 3186) was classified by whiskers and quartiles of C-peptide into four groups with the following ranges: 290-510 (n = 694), 511-710 (n = 780), 711-950 (n = 720) and 951-1560 pmol/l (n = 673). Fasting levels of glucose, insulin, HOMA IR (Homeostasis Model Assessment for Insulin Resistance) and BMI (body mass index) were compared by a relevant C-peptide range. Participants taking medication...
Sunitinib malate inhibits hemangioma cell growth and migration by suppressing focal adhesion kinase signaling
Wihan Scholtz, Peace Mabeta
J Appl Biomed 18:143-151, 2020 | DOI: 10.32725/jab.2020.019
Sunitinib malate is a small molecule that targets multiple receptor tyrosine kinases and blocks their activity. Receptors targeted by sunitinib are implicated in tumor vascularization and are overexpressed by vascular tumors encountered in infants, namely, hemangiomas. Of note is that there is still no definitive treatment for these commonly occurring tumors of infancy. The purpose of this study was to investigate the effects of sunitinib malate on hemangioma using endothelial cells isolated from a murine model of the neoplasm (sEnd.2). The effects of the drug on cell growth were evaluated using the crystal violet assay and flow cytometry, while the...