Journal of Applied Biomedicine, 2021 (vol. 19), issue 3

Acute effect of spinal cord stimulation on autonomic nervous system function in patients with heart failure

Jan Naar, Deborah Jaye, Petr Neužil, Petr Doškář, Filip Málek, Bengt Linderoth, Göran Lind, Marcus Ståhlberg

J Appl Biomed 19:133-141, 2021 | DOI: 10.32725/jab.2021.012  

Aims: To test the hypothesis that spinal cord stimulation (SCS) acutely improves heart rate variability (HRV) and baroreceptor sensitivity (BRS) in patients with heart failure (HF). Methods: SCS (15 minutes) was delivered in four different settings: 90% of maximal tolerated stimulation amplitude (MTA) targeting the T1-T4 spinal cord segments (SCS90T1-4), 60% of MTA (SCS60T1-4), 90% of MTA with cranial (SCS90CR) and caudal (SCS90CA) electrode configuration. HRV and BRS were recorded continuously and stimulation was compared to device off. Results: Fifteen HF patients were included. SCS90T1-4 did not change the standard deviation of intervals between...

Protective effect of sinomenine on isoproterenol-induced cardiac hypertrophy in mice

Le Li, Pu Fang, Jiekun Chen, Cailing Zhang, Houquan Tao

J Appl Biomed 19:142-148, 2021 | DOI: 10.32725/jab.2021.014  

To study the effect of sinomenine (Sin) on isoproterenol (Iso, β-agonist)-induced cardiac hypertrophy (CH), we set up four mouse groups: control, Iso model, Iso+metoprolol (Met, β blocker) 60 mg/kg and Iso+Sin 120 mg/kg. CH was induced by Iso (s.c. for 28 days) in mice, and Sin or Met were orally administered by gavage for 28 days in total. Left ventricular diastolic anterior wall thickness (LVAWd), left ventricular diastolic posterior wall thickness (LVPWd), left ventricular ejection fraction (LVEF), and short axis shortening (FS) were measured by echocardiography. Malondialdehyde (MDA) and total superoxide dismutase (T-SOD) were measured...

Effect of the natural flavonoids myricetin and dihydromyricetin on the wound healing process in vitro

Renáta Sklenářová, Marika Svrčková, Petr Hodek, Jitka Ulrichová, Jana Franková

J Appl Biomed 19:149-158, 2021 | DOI: 10.32725/jab.2021.017  

Myricetin (MYR) and dihydromyricetin (DHM) are classified as natural flavonoids. Both substances are known for their anti-inflammatory and antioxidant properties. In this study, an in vitro model of inflammation was demonstrated on monolayers of scratched fibroblasts or keratinocytes exposed to LPS from Pseudomonas aeruginosa for six hours. MYR and DHM were subsequently applied to the cells for 24 hours at sub toxic concentrations (5-15 µM). Inflammatory parameters were analysed in collected cell medium and lysate after the incubation period using the Enzyme-Linked ImmuneSorbent Assay (ELISA) and Western blot. Both flavonoids inhibit...

A-769662 stimulates the differentiation of bone marrow-derived mesenchymal stem cells into osteoblasts via AMP-activated protein kinase-dependent mechanism

Basem M. Abdallah, Abdullah M. Alzahrani

J Appl Biomed 19:159-169, 2021 | DOI: 10.32725/jab.2021.016  

AMP-activated protein kinase (AMPK) signaling shows an important role in energy metabolism and has recently been involved in osteogenic and adipogenic differentiation. In this study we aimed to investigate the role of AMPK activator, A-769662, in regulating the differentiation of mesenchymal stem cells derived from bone marrow (BMSCs) into osteoblastic and adipocytic cell lineage. The effect of A-769662 on osteogenesis was assessed by quantitative alkaline phosphatase (ALP) activity, matrix mineralization stained with Alizarin red, and gene expression analysis by quantitative polymerase chain reaction (qPCR). Adipogenesis was determined by Oil Red...

Protective effects of Dialium guineense pulp on aspirin-induced gastric mucosal injury in albino rats

Nwogo Ajuka Obasi, Chinyere Aloke, Stella Eberechukwu Obasi, Chinedu Ogbonnia Egwu, Ademola Clement Famurewa, Patience Nkemjika Ogbu

J Appl Biomed 19:170-178, 2021 | DOI: 10.32725/jab.2021.015  

The numerous challenges and detrimental effects connected with the treatment of peptic ulcers in the world today calls for alternative attention. Ethnomedicinally, Dialium guineense pulp (DAGP) has numerous pharmacological activities. This study investigated the anti-ulcer activities of Dialium guineense pulp on gastric mucosa injury induced with aspirin in albino Wistar rats. DAGP extract was orally administered at doses of 250, 500 and 1000 mg/kg bw (mg per kg of the body weight) per day for 3 or 7 days followed by 400 mg/kg bw oral aspirin administration. Ulcer indices were determined, followed by a biochemical estimation of antioxidant...

[Retracted] Does the enriched environment alter memory capacity in malnourished rats by modulating BDNF expression?

Waleska Maria Almeida Barros, Matheus Santos de Sousa Fernandes, Roberta Karlize Pereira Silva, Karollainy Gomes da Silva, Ana Patrícia da Silva Souza, Mariluce Rodrigues Marques Silva, Ana Beatriz Januário da Silva, Gabriela Carvalho Jurema Santos, Maria Eduarda Rodrigues Alves dos Santos, Taciane Silva do Carmo, Sandra Lopes de Souza, Viviane de Oliveira Nogueira Souza

J Appl Biomed 19:125-132, 2021 | DOI: 10.32725/jab.2021.018