Journal of Applied Biomedicine, 2022 (vol. 20), issue 1

Original Research Article

Overexpression of the miR-143/145 and reduced expression of the let-7 and miR-126 for early lung cancer diagnosis

Lubomír Tulinský, Anton Dzian, Tatiana Mataková, Peter Ihnát

J Appl Biomed 20:1-6, 2022 | DOI: 10.32725/jab.2022.004  

Introduction: Lung cancer is the leading cause of cancer-related deaths worldwide. For this reason, huge efforts are being invested in discovering suitable blood biomarkers that would allow early diagnosis and treatment. One of the possible promising candidates for this role are microRNA molecules (miRNAs). The aim of the study was to identify individual blood miRNAs that could be used as potential biomarkers for early diagnosis of lung cancer. Methods: This prospective study analyzed blood samples of 60 patients with early-stage lung cancer, and blood samples of 60 healthy individuals. All study patients with lung cancer had undergone radical pulmonary...

Evaluation of anti-biofilm, anti-quorum, anti-dysenteric potential of designed polyherbal formulation: in vitro and in vivo study

Devendra Singh, Virendra Singh, Shanti Bhushan Mishra, Deepmala Sharma, Vishnu Agarwal

J Appl Biomed 20:7-14, 2022 | DOI: 10.32725/jab.2022.005  

Bacillary dysentery (shigellosis) continues to cause havoc worldwide, with a high infectivity rate. It causes bloody diarrhea, and around 99% of bacillary dysentery cases occur in developing countries. The objective of this study is to develop a polyherbal formulation with the scientific rationale in treating infectious bacillary dysentery disease. The anti-bacterial activity, the minimum inhibitory concentration of the formulation against bacillary dysentery, causing microbes like Shigella flexneri (MTCC 1457), Escherichia coli (MTCC 1687), and Salmonella enterica (MTCC 98), was analysed by well-diffusion method and broth dilution...

Evaluation of anti-methicillin-resistant Staphylococcus aureus property of zerumbone

Shaymaa Fadhel Abbas Albaayit, Rukesh Maharjan, Rasedee Abdullah, Mohd Hezmee Mohd Noor

J Appl Biomed 20:15-21, 2022 | DOI: 10.32725/jab.2022.002  

Context and objective: Zerumbone has been reported to exert anti-microbial effects, but the mechanism by which the compound exerts its action is not known. Thus, this study aimed to investigate the mechanism of action of zerumbone against methicillin-resistance Staphylococcus aureus (MRSA), using the atomic force microscopy (AFM), scanning electron microscopy (SEM), and flow cytometry techniques. Methods: MRSA (NCTC 13277) cell viability was determined using the microplate AlamarBlue assay. AFM and SEM were used to determine the morphology of zerumbone-treated MRSA cells. Flow cytometric analysis was used to determine the effect of zerumbone...

Exploring the potential of Cinnamomum zeylanicum oil against drug resistant Helicobacter pylori-producing cytotoxic genes

Sameh S. Ali, Manar K. Abd Elnabi, Mohammad M. Alkherkhisy, Abdulkarim Hasan, Fanghua Li, Maha Khalil, Jianzhong Sun, Nessma El-Zawawy

J Appl Biomed 20:22-36, 2022 | DOI: 10.32725/jab.2022.003  

Thirty-one of sixty dyspeptic patients tested positive for Helicobacter pylori colonization in this study, as determined by histopathology and 16S rRNA. The cytotoxin-associated gene A (cagA) and vacuolating cytotoxin A (vacA) genes were found in 67.7 and 93.5% of H. pylori patients, respectively. The cagA gene was found to be associated with 100% of patients with duodenal erosion and ulceration identified via endoscopy examination. In addition, 86.7% of patients with cancerous and precancerous lesions, glandular atrophy, and intestinal metaplasia identified via histopathology examination. The vacA s1m1...

Association between interferon-gamma (IFN-γ) gene polymorphisms (+874A/T and +2109A/G), and susceptibility to hepatitis B viral infection (HBV)

Mahmoud F. Dondeti, Mohamed S. Abdelkhalek, Hosam El-Din M Elezawy, Walaa F. Alsanie, Bassem M. Raafat, Amira M. Gamal-Eldeen, Roba M. Talaat

J Appl Biomed 20:37-43, 2022 | DOI: 10.32725/jab.2022.001  

Background: Interferon-gamma (IFN-γ) is a chief proinflammatory cytokine with a significant role in the immune response against viral infections. Today there is increasing evidence about the association between individual genetic polymorphisms and cytokines in predicting HBV infection susceptibility. Aim: This study aimed to investigate the association between IFN-γ gene polymorphisms and susceptibility to hepatitis B viral infection (HBV), and the impact of these genetic polymorphisms on IFN-γ production. IFN-γ (+874A/T, rs2430561, and +2109A/G, rs1861494) was genotyped by single-stranded polymorphism-polymerase chain reaction...