Journal of Applied Biomedicine, 2024 (vol. 22), issue 4
Original Research Article
Linoleic acid inhibits lipopolysaccharide-induced inflammation by promoting TLR4 regulated autophagy in murine RAW264.7 macrophages
Yin Qin, Kexin Li, Qiuhong Zhang, Jie Liu, Yu Xie, Tingting Zhang, Xiaoliang Wang, Li Zhang, Yu Jiang, Gang Liu
J Appl Biomed 22:185-196, 2024 | DOI: 10.32725/jab.2024.023 
Linoleic acid (LA), an essential fatty acid, has emerged as a pivotal regulator in disorders associated with inflammation in recent years; however, the underlying mechanisms are still not completely understood. We utilized network pharmacology and experimental methodologies to elucidate the mechanisms underlying the anti-inflammatory effects of LA. Our network pharmacology analysis revealed that LA shares common targets with sepsis. These targets are enriched in various pathways comprising C-type signaling pathway, PI3K-Akt signaling pathway, toll-like receptor signaling pathway, neutrophil extracellular trap formation, AMPK signaling pathway, and...
Syringin protects high glucose-induced BMSC injury, cell senescence, and osteoporosis by inhibiting JAK2/STAT3 signaling
Yu-Cong Zou, Kai Gao, Bao-Tao Cao, Xiao-Li He, Wei Zheng, Xiao-Fei Wang, Yu-Fu Li, Feng Li, Hua-Jun Wang
J Appl Biomed 22:197-207, 2024 | DOI: 10.32725/jab.2024.021
Background: Acanthopanax senticosus (Rupr. et Maxim.) is commonly used in Traditional Chinese Medicine. Syringin is a major ingredient of phenolic glycoside in Acanthopanax senticosus. Objective: This study was performed to investigate whether Syringin could protect high glucose-induced bone marrow mesenchymal stem cells (BMSCs) injury, cell senescence, and osteoporosis by inhibiting JAK2/STAT3 signaling. Methods: BMSCs isolated from both the tibia and femur of mice were induced for osteogenesis. The cell senescence was induced using the high glucose medium. The cells were treated with 10 and 100 μmol/l Syringin. Immunohistochemistry staining...
Association between mean platelet volume and adiponectin in patients with metabolic syndrome
Milos Marinkovic, Jelena Nesic, Ivica Petrovic, Ivan P Jovanovic, Marija Sekulic, Svetlana Djukic, Aleksandar Djukic
J Appl Biomed 22:208-213, 2024 | DOI: 10.32725/jab.2024.022
Background: Metabolic syndrome is a significant pro-inflammatory and pro-coagulant condition. The clinical association of adiponectin, a mainly antidiabetogenic molecule, and its interaction with platelets and platelet indices remains insufficiently investigated. Objective: The aim of our study was to investigate the association of adiponectin with platelets and platelet indices in patients with metabolic syndrome. Methods: The investigation was conducted as a cross-sectional study involving 113 subjects: 63 patients with the diagnosis of metabolic syndrome, and 50 healthy controls - with clear inclusion and exclusion criteria. The group of patients...
BRAF V600E positive papillary thyroid carcinoma (TERT and TP53 mutation coexistence excluded): Correlation of clinicopathological features and the extent of surgical treatment and its complications
Jiří Hložek, Jan Rotnágl, Richard Holý, Tereza Hložková, Barbora Peková Bulanová, Vlasta Kuklíková, Běla Bendlová, Jiří Soukup, Petr Hrabal, Jaromír Astl
J Appl Biomed 22:214-220, 2024 | DOI: 10.32725/jab.2024.025
Introduction: Papillary thyroid carcinoma (PTC) frequently harbors the BRAF V600E mutation. Recent research suggests that aggressive behavior in BRAF V600E+ PTC may be due to an undetected mutation in the TERT gene. This study aims to observe the clinicopathological features of BRAF V600+ PTC and correlate them with surgical treatment complications. Methods: A retrospective analysis was conducted on the BRAF V600E+ PTC cohort from July 2019 to January 2023. The histopathological features and surgical treatment (total thyroidectomy - group A, total thyroidectomy + central block neck dissection - group B) complications...
Endoscopic luminal impedance planimetry of the lower oesophageal sphincter and pylorus in experimental pigs: a pilot study
Jan Bureš, Věra Radochová, Darina Kohoutová, Miroslav Zavoral, Kristina Hugová, Štěpán Suchánek, Ondřej Soukup, Jan Martínek
J Appl Biomed 22:221-227, 2024 | DOI: 10.32725/jab.2024.026
Background/Aims: The functional lumen imaging probe (FLIP) relies on the principle of impedance planimetry that enables direct measurement of intraluminal pressure, cross-sectional areas, and wall biomechanical properties. The aim of our pilot project was to introduce this method to assess function of the lower oesophageal sphincter and pyloric muscle in experimental pigs. Methods: All measurements were accomplished in one session in six adult female pigs (mean weight 34.2 ± 3.6 kg), using the EndoFLIP 1.0 System with EndoFLIP catheters. Five major parameters were evaluated: balloon pressure (mm Hg), estimated diameter (mm), cross-sectional area...
Effects of different hemodialysis modalities combined with low-calcium dialysate on mineral metabolism and vascular calcification in maintenance hemodialysis patients with chronic kidney disease
Jing Wang, Yimian Luo, Xingyu Ji, Hao Xu, Zhenhua Liang, Minjie Zhou
J Appl Biomed 22:228-233, 2024 | DOI: 10.32725/jab.2024.027
Objective: This research investigated the effects of different hemodialysis modalities combined with low-calcium dialysate (LCD) on mineral metabolism and vascular calcification (VC) in maintenance hemodialysis (MHD) patients with chronic kidney disease (CKD). Methods: General data were collected from 192 cases of MHD patients, who were divided into 4 groups according to the randomized numerical table. Each group was given LCD treatment, and conventional hemodialysis (HD), high-flux HD (HFHD), hemodiafiltration (HDF), and HD + hemoperfusion (HP) were performed, respectively. The patients were dialyzed 3 times per week for 4 h each time, and each group...
Review Article
The role of chemokines and interleukins in acute lymphoblastic leukemia: a systematic review
Martin Štajer, Jan M. Horáček, Tomáš Kupsa, Pavel Žák
J Appl Biomed 22:165-184, 2024 | DOI: 10.32725/jab.2024.024
Acute lymphoblastic leukemia (ALL) is the most common childhood hematological malignancy, but it also affects adult patients with worse prognosis and outcomes. Leukemic cells benefit from protective mechanisms, which are mediated by intercellular signaling molecules - cytokines. Through these signals, cytokines modulate the biology of leukemic cells and their surroundings, enhancing the proliferation, survival, and chemoresistance of the disease. This ultimately leads to disease progression, refractoriness, and relapse, decreasing the chances of curability and overall survival of the patients. Targeting and modulating these pathological processes without...