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- published from 2003 – freely available (since 2019 + 2003-2013), published by Elsevier 2014-2018
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Recent achievements
Two our publications got the Award of the Czech Society of Otorhinolaryngology and Head and Neck Surgery for the best publication in 2024
Latest articles
Results 1 to 30 of 68:
Secondary metabolites from halotolerant filamentous fungi as potential topical cosmeceutical ingredientsOriginal Research Article
Chi Hoang, Ha Tran, Hang Tran, Diep Hoang, Quan Nguyen, Cuong Le
J Appl Biomed 23:152-162, 2025 | DOI: 10.32725/jab.2025.013 
The use of natural products in cosmetics and pharmacy has risen dramatically in recent years, leading to the overexploitation of flora and fauna worldwide and threatening the environmental sustainability. Microbe-derived components could help to solve the problem due to their independently controllable cultural property. To investigate the potential of microfungi for producing potential novel cosmeceuticals, cerevisterol (1), aloesol (2), 3β,5α,9α-trihydroxyergosta-7,22-diene-6-one (3), and ergosterol peroxide (4) were isolated from the halotolerant fungal strains Penicillium brefeldianum CL6 and Talaromyces...
Influence of the antidiabetic drug metformin on the aquatic crustacean Daphnia magnaOriginal Research Article
Martina Poncarová, Šárka Klementová, Michal Šorf
J Appl Biomed 23:144-151, 2025 | DOI: 10.32725/jab.2025.011
Background: The antidiabetic drug metformin has been repeatedly detected in surface waters worldwide. This study investigates the effects of the environmentally relevant concentration of metformin on a non-target aquatic organism - a freshwater crustacean, Daphnia magna, with an emphasis on the stress response of daphnids and the long-term effects on their consecutive generations. Methods: The chronic toxicity test and the consecutive generations test were inspired by the OECD method. The total antioxidant capacity (Trolox equivalent - TEAC), superoxide dismutase (SOD) activity, and catalase (CAT) activity were related to the protein content...
Applying the technology ORBEYETM exoscope in transoral exoscopic laryngeal surgery: single centre prospective studyOriginal Research Article
Richard Holý, Tomáš Filipovský, Eva Lukavcová, David Kalfeřt, Pavol Praženica, Petra Dytrych, Jiří Hložek, Jan Rotnágl, Daniel Kovář, Jaromír Astl
J Appl Biomed 23:138-143, 2025 | DOI: 10.32725/jab.2025.010
Introduction: ORBEYETM exoscope offers superior visualization with clearer imaging compared to standard methods and supports narrow band imaging (NBI). ORBEYETM provides better visualization of tissue structures, thus increasing the accuracy of the surgical procedure. The systematic use of ORBEYETM in transoral exoscopic laryngeal surgery (TOLES) is rarely documented. This study evaluates the sensitivity and specificity of TOLES using ORBEYETM with white light and NBI modalities. Methods: Between 8/2021 and 8/2024, 84 patients underwent TOLES using ORBEYETM with white light and NBI modes. All surgeries...
Epidemiology of late postoperative bleeding in OSA-related tonsil surgery: a multicentric retrospective studyOriginal Research Article
Jan Vodička, Martin Chovanec, Milan Urík, Břetislav Gál, Rami Katra, Petr Škopek, Veronika Glumbíková, Anna Švejdová, Zdeněk Knížek, Jan Kolín, Hana Doležalová, Libor Sychra, Patrik Bursa, Lenka Jetmarová, Silvia Berková, Pavel Strejček, Josef Hájek, Tomáš Kostlivý, David Slouka
J Appl Biomed 23:126-137, 2025 | DOI: 10.32725/jab.2025.016
Introduction: Tonsil-related procedures are considered fundamental and effective in the surgical treatment of obstructive sleep apnea (OSA). The range of techniques includes intratonsillar approaches, such as tonsillotomy (TT), as well as extracapsular procedures, such as tonsillectomy (TE) and uvulopalatopharyngoplasty (UPPP). Patients undergoing these procedures span all age groups, from children to seniors. Methods: This multicentric retrospective study, conducted between 2014 and 2018, analysed data from 3,498 patients who underwent bilateral TT, TE, or UPPP for OSA or ronchopathy. The cohort included 2,221 men (63.49%) and 1,277 women (36.51%)....
Characteristics of healthy sinonasal microbiome - single-centre study in the Czech RepublicOriginal Research Article
Kristýna Mamiňák, Karla Janoušková, Richard Holý, Alžběta Prášilová, Tomáš Filipovský, Daniel Kovář, Jaromír Astl
J Appl Biomed 23:117-125, 2025 | DOI: 10.32725/jab.2025.012
Introduction: The human nasal cavity and paranasal sinuses host a complex and dynamic microbiome which has a crucial role in mucosal immunity. A comprehensive profile of the healthy sinonasal microbiome remains limited. The purpose of our study was to characterize the healthy sinonasal microbiome in adults using 16S rRNA long-read sequencing to enable species-level resolution, and to assess its associations with demographical and clinical factors such as smoking, allergy history, and olfactory function. Study design: We performed a prospective, single-centre study analysing middle meatus samples from 27 healthy individuals undergoing septoplasty in...
Serum xanthine oxidoreductase and oxidative stress are associated with bladder cancer: a case-control study from JordanOriginal Research Article
Lina Elsalem, Abrar A. Aleikish, Nosayba Al-Azzam, Mahmoud A. Alfaqih, Haneen A. Basheer, Omar Halalsheh
J Appl Biomed 23:107-116, 2025 | DOI: 10.32725/jab.2025.015
Xanthine oxidoreductase (XOR) is an oxidant enzyme that exists mainly in two distinct forms: the dehydrogenase form [xanthine dehydrogenase (XDH)] and the oxidized form [xanthine oxidase (XO)]. XO might contribute to tumorigenesis through direct metabolic activation of carcinogens and indirect generation of free radicals. Oxidative stress is one of the leading causes of bladder cancer (BC). Smoking and genetic susceptibility are also linked to oxidative stress and BC. This study investigated the association between XO serum levels and XOR genetic polymorphisms with BC. A case-control study was conducted among 109 BC patients and 109 controls...
Elevated plasma levels of cell-free mtDNA are associated with acute rejection following heart transplantationOriginal Research Article
Dana Dlouhá, Kristýna Janoušková, Šárka Chytilová, Jevgenija Vymětalová, Marianna Lukášová, Šárka Nováková, Eva Rohlová, Jaroslav A. Hubáček
J Appl Biomed 23:97-106, 2025 | DOI: 10.32725/jab.2025.014
Acute rejection (AR) following heart transplantation (HTx) is a common complication, especially in the early post-HTx period. Mitochondrial DNA (mtDNA), released into circulation from stressed mitochondria, mimics ongoing immune activation and facilitates the release of pro-inflammatory substances. Our study aimed to assess cell-free mtDNA levels to identify early indicators of acute rejection progression. The absolute concentration of cf-mtDNA (cp/μl) was measured in 77 adult patients using quantitative polymerase chain reaction. Blood samples (n = 300) were collected before their corresponding biopsy according to the timeline within the...
Incidence of congenital microcephaly in the Czech Republic: The effect of maternal ageOriginal Research Article
Natálie Friedová, Antonín Šípek, Jan Klaschka, Marek Malý, Pavel Calda, Frantisek Liška, Antonín Šípek, Jr.
J Appl Biomed 23:91-95, 2025 | DOI: 10.32725/jab.2025.009
Objective: Congenital microcephaly is a diverse group of congenital anomalies characterized by a significantly reduced head circumference at birth. The incidence varies widely across regions. This study focuses on the incidence of microcephaly in the Czech Republic. Methods: A retrospective analysis was conducted using data from the National Registry of Congenital Anomalies of the Czech Republic. All cases coded as microcephaly (Q02 code in the 10th revision of the International Classification of Diseases) between 2000 and 2020 were included. Results: A total of 274 cases of congenital microcephaly were identified, with an incidence rate of 1.22 per...
Unlocking the secrets of aortic pseudoaneurysms - exploring tensile testing of prostheses, anastomoses, and native vessels in the thoracic aorta: A clinical-engineering correlationOriginal Research Article
Sandra Rečičárová, Hynek Chlup, Michael Jonák, Ivan Netuka
J Appl Biomed 23:80-90, 2025 | DOI: 10.32725/jab.2025.008
Introduction: This study examines the mechanical properties of thoracic aortic false aneurysms (TAFA) and how the use of vascular prostheses, native vessels, and anastomoses affects their development. This is done through tensile testing, simulating a Bentall procedure, which is the most common surgery leading to TAFA development. Methods: We conducted uniaxial tensile tests on the native right and left coronary arteries from five cadaveric donors. They were anastomosed to two vascular prostheses in the longitudinal and circumferential directions to assess their mechanical responses under load. Results and discussion: All anastomosis specimens ruptured...
Berberine improves atrial remodeling by regulating the AMPK/PPARα signaling pathway in a rabbit model of atrial fibrillationOriginal Research Article
Yang Wang *, Zhe Sun, Zong-tao Yin, Jian Zhang, Fang-ran Xin, Yin-li Xu, Huai Lan
J Appl Biomed 23:63-79, 2025 | DOI: 10.32725/jab.2025.007
Atrial fibrillation (AF) is a common arrhythmia encountered in clinical practice, characterized by myocardial fibrosis and atrial remodeling as its primary pathological features, and associated with significantly high mortality and disability rates. Currently, there are no specific pharmacological treatments for AF, and traditional anti-arrhythmic drugs have not achieved the desired efficacy, often resulting in a high incidence of adverse drug reactions. Thus, there is an urgent need for the development of novel anti-AF medications. Berberine, the main active component of Coptis chinensis, has been shown to have antiarrhythmic and anti-heart...
The importance of olfactory and trigeminal event-related potentials (OERPs/TERPs) in the assessment of olfactory function in subjects with chronic rhinosinusitis with nasal polyposisOriginal Research Article
Karla Janoušková, Oleksii Vorobiov, Kristýna Mamiňák, David Kalfeřt, Libor Vašina, Petra Dytrych, Nikola Pastorková, Jiří Hložek, Daniel Kovář, Jan Vodička, Václav Masopust, Jaromír Astl, Richard Holý
J Appl Biomed 23:57-62, 2025 | DOI: 10.32725/jab.2025.006
Objectives: Smell impairment (SI) is a well-known symptom of chronic rhinosinusitis with nasal polyps (CRSwNP). The aim of study was to analyze olfactory and trigeminal event-related potentials (OERPs/TERPs) and psychophysical smell tests in subjects with CRSwNP, and its potential role in clinical practice. Methods: Prospective study included 57 subjects. Two investigated groups with CRSwNP and one control group. Group I (n = 20) contained subjects without CRSwNP before septoplasty. Group II (n = 18) contained subjects with CRSwNP without therapy. Group III (n = 19) contained subjects with CRSwNP after intranasal corticosteroid...
Seleno-chitooligosaccharide-induced modulation of intestinal barrier function: Role of inflammatory cytokines, tight junction proteins, and gut microbiota in miceOriginal Research Article
Zhuo-Hong He, Ye Jin, Die Chen, Hui-Xin Zheng, Jia-Er Xiang, Yong-Jun Jiang, Zheng-Shun Wen
J Appl Biomed 23:45-55, 2025 | DOI: 10.32725/jab.2025.003
This study aimed to explore the function of Seleno-chitooligosaccharide (SOA) on the intestinal barrier through regulation of inflammatory cytokines, tight junction protein, and gut microbiota in mice. The results of ELISA assay demonstrated that SOA significantly increased the levels of IL-2, IL-10, and IFN-γ in serum and ileum. Meanwhile, SOA increased the levels of IL-4 in the ileum (p < 0.05). In addition, Diamine Oxidase (DAO) concentration was decreased in ileum by SOA treatments (p < 0.05). The administration of SOA significantly upregulated the expression of ZO-1 and Occludin in the ileum (p < 0.05). By 16S...
Distribution of human leukocyte antigen B27 (HLA-B27) in Slovak patientsOriginal Research Article
Alexandra Pôbišová, Andrea Kollárová, Vladimír Langraf, František Strejček, Ramona Babosová
J Appl Biomed 23:36-44, 2025 | DOI: 10.32725/jab.2025.004
Background and objectives: HLA-B27 is a genetic marker associated with spondyloarthropathies, particularly ankylosing spondylitis and axial spondyloarthritis. While its prevalence varies across populations, no data exist for Slovak patients. This study aimed to determine HLA-B27 prevalence in Slovak patients with suspected spondyloarthropathies and assess differences by sex and age. Methods: A retrospective cohort of 1,614 patients (888 females and 726 males) was analyzed for HLA-B27 status (positive/negative) using reverse hybridisation (HLA-B27 StripAssay). Statistical analyses included Pearson's Chi-square test and non-parametric Mann-Whitney U...
Changes in glucose-related parameters according to LDL-cholesterol concentration ranges in non-diabetic patientsOriginal Research Article
Vladimír Kron, Miroslav Verner, Pavel Smetana, Radka Vrzalová, Eliška Friebergerová, Daniel Martiník, Dagmar Horáková, Ladislav Štěpánek, Lidmila Hamplová, Kvetoslava Kotrbová
J Appl Biomed 23:26-35, 2025 | DOI: 10.32725/jab.2025.005
The study focused on the changes in C-peptide, glycemia, insulin concentration, and insulin resistance according to LDL-cholesterol concentration ranges. The metabolic profile of individuals in the Czech Republic (n = 1840) was classified by quartiles of LDL-cholesterol into four groups with the following ranges: 0.46-2.45 (n = 445), 2.46-3.00 (n = 474), 3.01-3.59 (n = 459), and 3.60-7.18 mmol/l (n = 462). The level of glucose, C-peptide, insulin, and area of parameters during OGTT and HOMA IR were compared with a relevant LDL-cholesterol range. The evaluation involved correlations between LDL-cholesterol and the...
Chemical composition and anticancer activity of Psychotria montana on MCF7 breast cancer cells: insights from in vitro (2D & 3D) studies and in silico analysisOriginal Research Article
Van Hung Hoang, Thi Kieu Oanh Nguyen, Phu Hung Nguyen, Thi Thanh Huong Le, Viet Hoang
J Appl Biomed 23:12-25, 2025 | DOI: 10.32725/jab.2025.002
Aim: This study aimed to investigate the phytochemical composition of Psychotria montana extract (PME) and evaluate its inhibitory effects on MCF7 breast cancer cells. Methods: The chemical composition of PME was analyzed using UPLC-QToF-MS. The effects of PME on cell proliferation were evaluated using the MTT assay. Flow cytometry was used for cell cycle and apoptosis analysis. The effects of PME on the transcription of cell cycle control genes were assessed using real-time PCR. Results: UPLC-QToF-MS analysis revealed major compounds of PME, including terpenoids and flavonoids, with the potential to inhibit proliferation, migration, and induce...
Systematic review of antibacterial potential in calcium oxide and silicon oxide nanoparticles for clinical and environmental infection controlReview Article
Hend Algadi, Mohammed Abdelfatah Alhoot, Laith A. Yaaqoob
J Appl Biomed 23:1-11, 2025 | DOI: 10.32725/jab.2025.001
A substantial threat to worldwide health, the proliferation of antibiotic-resistant bacteria compels researchers to seek innovative antibacterial substances. This systematic review assesses the role of nanoparticles, particularly Calcium oxide and Silicon oxide nanoparticles, in infection control. The article examines the mechanisms by which these nanoparticles act against various bacteria and evaluates their potential as novel agents in infection control strategies. A systematic literature search from 2015 to 2024 encompassing Web of Science, PubMed, Wiley, Science Direct, and Google Scholar, yielded 70 publications meeting the review criteria. This...
Effects of different hemodialysis modalities combined with low-calcium dialysate on mineral metabolism and vascular calcification in maintenance hemodialysis patients with chronic kidney diseaseOriginal Research Article
Jing Wang, Yimian Luo, Xingyu Ji, Hao Xu, Zhenhua Liang, Minjie Zhou
J Appl Biomed 22:228-233, 2024 | DOI: 10.32725/jab.2024.027
Objective: This research investigated the effects of different hemodialysis modalities combined with low-calcium dialysate (LCD) on mineral metabolism and vascular calcification (VC) in maintenance hemodialysis (MHD) patients with chronic kidney disease (CKD). Methods: General data were collected from 192 cases of MHD patients, who were divided into 4 groups according to the randomized numerical table. Each group was given LCD treatment, and conventional hemodialysis (HD), high-flux HD (HFHD), hemodiafiltration (HDF), and HD + hemoperfusion (HP) were performed, respectively. The patients were dialyzed 3 times per week for 4 h each time, and each group...
Endoscopic luminal impedance planimetry of the lower oesophageal sphincter and pylorus in experimental pigs: a pilot studyOriginal Research Article
Jan Bureš, Věra Radochová, Darina Kohoutová, Miroslav Zavoral, Kristina Hugová, Štěpán Suchánek, Ondřej Soukup, Jan Martínek
J Appl Biomed 22:221-227, 2024 | DOI: 10.32725/jab.2024.026
Background/Aims: The functional lumen imaging probe (FLIP) relies on the principle of impedance planimetry that enables direct measurement of intraluminal pressure, cross-sectional areas, and wall biomechanical properties. The aim of our pilot project was to introduce this method to assess function of the lower oesophageal sphincter and pyloric muscle in experimental pigs. Methods: All measurements were accomplished in one session in six adult female pigs (mean weight 34.2 ± 3.6 kg), using the EndoFLIP 1.0 System with EndoFLIP catheters. Five major parameters were evaluated: balloon pressure (mm Hg), estimated diameter (mm), cross-sectional area...
BRAF V600E positive papillary thyroid carcinoma (TERT and TP53 mutation coexistence excluded): Correlation of clinicopathological features and the extent of surgical treatment and its complicationsOriginal Research Article
Jiří Hložek, Jan Rotnágl, Richard Holý, Tereza Hložková, Barbora Peková Bulanová, Vlasta Kuklíková, Běla Bendlová, Jiří Soukup, Petr Hrabal, Jaromír Astl
J Appl Biomed 22:214-220, 2024 | DOI: 10.32725/jab.2024.025
Introduction: Papillary thyroid carcinoma (PTC) frequently harbors the BRAF V600E mutation. Recent research suggests that aggressive behavior in BRAF V600E+ PTC may be due to an undetected mutation in the TERT gene. This study aims to observe the clinicopathological features of BRAF V600+ PTC and correlate them with surgical treatment complications. Methods: A retrospective analysis was conducted on the BRAF V600E+ PTC cohort from July 2019 to January 2023. The histopathological features and surgical treatment (total thyroidectomy - group A, total thyroidectomy + central block neck dissection - group B) complications...
Association between mean platelet volume and adiponectin in patients with metabolic syndromeOriginal Research Article
Milos Marinkovic, Jelena Nesic, Ivica Petrovic, Ivan P Jovanovic, Marija Sekulic, Svetlana Djukic, Aleksandar Djukic
J Appl Biomed 22:208-213, 2024 | DOI: 10.32725/jab.2024.022
Background: Metabolic syndrome is a significant pro-inflammatory and pro-coagulant condition. The clinical association of adiponectin, a mainly antidiabetogenic molecule, and its interaction with platelets and platelet indices remains insufficiently investigated. Objective: The aim of our study was to investigate the association of adiponectin with platelets and platelet indices in patients with metabolic syndrome. Methods: The investigation was conducted as a cross-sectional study involving 113 subjects: 63 patients with the diagnosis of metabolic syndrome, and 50 healthy controls - with clear inclusion and exclusion criteria. The group of patients...
Syringin protects high glucose-induced BMSC injury, cell senescence, and osteoporosis by inhibiting JAK2/STAT3 signalingOriginal Research Article
Yu-Cong Zou, Kai Gao, Bao-Tao Cao, Xiao-Li He, Wei Zheng, Xiao-Fei Wang, Yu-Fu Li, Feng Li, Hua-Jun Wang
J Appl Biomed 22:197-207, 2024 | DOI: 10.32725/jab.2024.021
Background: Acanthopanax senticosus (Rupr. et Maxim.) is commonly used in Traditional Chinese Medicine. Syringin is a major ingredient of phenolic glycoside in Acanthopanax senticosus. Objective: This study was performed to investigate whether Syringin could protect high glucose-induced bone marrow mesenchymal stem cells (BMSCs) injury, cell senescence, and osteoporosis by inhibiting JAK2/STAT3 signaling. Methods: BMSCs isolated from both the tibia and femur of mice were induced for osteogenesis. The cell senescence was induced using the high glucose medium. The cells were treated with 10 and 100 μmol/l Syringin. Immunohistochemistry staining...
Linoleic acid inhibits lipopolysaccharide-induced inflammation by promoting TLR4 regulated autophagy in murine RAW264.7 macrophagesOriginal Research Article
Yin Qin, Kexin Li, Qiuhong Zhang, Jie Liu, Yu Xie, Tingting Zhang, Xiaoliang Wang, Li Zhang, Yu Jiang, Gang Liu
J Appl Biomed 22:185-196, 2024 | DOI: 10.32725/jab.2024.023
Linoleic acid (LA), an essential fatty acid, has emerged as a pivotal regulator in disorders associated with inflammation in recent years; however, the underlying mechanisms are still not completely understood. We utilized network pharmacology and experimental methodologies to elucidate the mechanisms underlying the anti-inflammatory effects of LA. Our network pharmacology analysis revealed that LA shares common targets with sepsis. These targets are enriched in various pathways comprising C-type signaling pathway, PI3K-Akt signaling pathway, toll-like receptor signaling pathway, neutrophil extracellular trap formation, AMPK signaling pathway, and...
The role of chemokines and interleukins in acute lymphoblastic leukemia: a systematic reviewReview Article
Martin Štajer, Jan M. Horáček, Tomáš Kupsa, Pavel Žák
J Appl Biomed 22:165-184, 2024 | DOI: 10.32725/jab.2024.024
Acute lymphoblastic leukemia (ALL) is the most common childhood hematological malignancy, but it also affects adult patients with worse prognosis and outcomes. Leukemic cells benefit from protective mechanisms, which are mediated by intercellular signaling molecules - cytokines. Through these signals, cytokines modulate the biology of leukemic cells and their surroundings, enhancing the proliferation, survival, and chemoresistance of the disease. This ultimately leads to disease progression, refractoriness, and relapse, decreasing the chances of curability and overall survival of the patients. Targeting and modulating these pathological processes without...
In vitro biological activities of Calamintha nepeta L. aqueous extractsOriginal Research Article
Nadia Salhi, Dorien Deluyker, Virginie Bito, Abdelhamid Zaid, Lhoussaine El Rhaffari
J Appl Biomed 22:155-163, 2024 | DOI: 10.32725/jab.2024.017
Aim: This study aimed to investigate the phenolic composition, antioxidant capacity, and toxicity of aqueous extracts of Calamintha nepeta L. leaves and their potential vasorelaxant effects. Methods: Aqueous extracts of Calamintha nepeta L. were prepared by three extraction methods: decoction, infusion, and maceration. The total phenolic contents of the extracts and their antioxidant properties were investigated. The toxicity was evaluated by Artemia salina lethality bioassay. The decoction extract was analyzed by HPLC for its chemical profile and was also used to evaluate the vasorelaxant effect on thoracic aortic rings isolated...
Olfactory event-related potentials (OERPs) and trigeminal event-related potentials (TERPs) in subjects after Covid-19 infection: single-center prospective studyOriginal Research Article
Richard Holý, David Kalfeřt, Libor Vašina, Oleksii Vorobiov, Petra Dytrych, Karla Janoušková, Eva Augste, Shahriar Kashiri, Nikola Pastorková, Kristýna Mamiňák, Jiří Hložek, Daniel Kovář, Jan Vodička, Jaromír Astl
J Appl Biomed 22:149-154, 2024 | DOI: 10.32725/jab.2024.020
Objectives: Olfactory dysfunction (OD) is a common symptom associated with Covid-19. During the Covid-19 pandemic, the importance of psychophysical olfactory tests and electrophysiological olfactory assessment increased. The purpose of the study was to analyze the psychophysical olfactory tests and the post-covid curves of olfactory event-related potentials (OERPs) and trigeminal event-related potentials (TERPs). Methods: The prospective study included 98 subjects (62 females / 36 males). The average age was 42 years (range 21-84 years). Group I (n = 77) contained participants who had been infected with Covid-19. They were enrolled in the study...
Salivary glands - a new site of Helicobacter pylori occurrenceOriginal Research Article
Jan Rotnágl, Jiří Hložek, Richard Holý, Emil Pavlík, David Kalfeřt, Jaromír Astl
J Appl Biomed 22:141-148, 2024 | DOI: 10.32725/jab.2024.018
Objective: The role of Helicobacter pylori (Hp) in the pathological processes of the gastric mucosa is well understood. Decreasing trend in successful eradication of HP from the stomach was observed in last years. This lack of succes is mainly caused by increasing ATB resistance. Nevertheless other possible causes of this phenomenon are being explored. Thus, many studies have focused on the search for extragastric reservoirs as potential sources of persistence or reinfection after successful Hp eradication. The pathological potential of Hp at these localities has also been studied. Methods: Our study aimed to determine the presence of Hp inside...
Anti-NMDAR1 antibody impairs dendritic branching in immature cultured neuronsOriginal Research Article
Pascal Jorratt, Aneta Petrušková
J Appl Biomed 22:136-140, 2024 | DOI: 10.32725/jab.2024.019
Anti-N-methyl D-aspartate receptor (anti-NMDAR) encephalitis is an autoimmune disorder characterized by IgG antibodies targeting NMDAR. The prevalence is remarkably higher in women and some develop the condition during pregnancy. While immunotherapies have shown good outcomes for pregnant mothers and their infants, the impact on early neurodevelopment remains elusive. This study investigates the effects of anti-NMDAR antibody on the development of primary cortical cultures. Anti-NMDAR antibody was administered to the cultures at day in vitro 5 for the following 5 days to assess dendritic branching and arbor complexity, and at day in...
Astragaloside IV confronts amyloid-beta-induced astrocyte senescence via hsp90aa1Original Research Article
Xia Yan, Rongxiang Zeng, Yajun Cao
J Appl Biomed 22:129-135, 2024 | DOI: 10.32725/jab.2024.015
Cell senescence is intensively related to aging and neurodegenerative diseases. This study aimed to explore the effect and targets of Astragaloside IV against amyloid-beta-induced astrocyte senescence. Oligomerized amyloid-beta was prepared to culture with human astrocytes. The effects of Astragaloside IV were assessed based on SA-β-gal staining analysis, senescence markers (p53, p16INK4, and p21WAF1), neurotrophic growth factor levels (qRT-PCR), and cell proliferation (CCK-8 kit). The targets for Astragaloside IV were predicted, and hsp90aa1 protein was verified using molecular docking. After hsp90aa1 overexpression, the effects of Astragaloside...
Today's cancer research and treatment - highly sophisticated and molecularly targeted, yet firmly bolstered in the classical theoriesReview Article
Thomas W. Grunt
J Appl Biomed 22:123-128, 2024 | DOI: 10.32725/jab.2024.016
Cancer research is linked to modern life-sciences, encompassing achievements in virology, yeast-biology, molecular-biology, genetics, systems-biology, bioinformatics, and so on. With these fascinating developments, it's easy to overlook that the fundamental theories and treatment strategies were established in the early 20th century and have remained valid ever since. Therefore, tribute must be paid to the founders of the field. The main hypotheses on carcinogenesis, the genetic model and the metabolic model, and the concept of cancer-treatment with cytotoxic, targeted or metabolic drugs were proposed more than 100 years ago by great minds such as...
RT-qPCR investigation of post-mortem tissues during COVID-19Original Research Article
Zhanna Berdygulova, Elina Maltseva, Yuliya Perfilyeva, Anna Nizkorodova, Andrey Zhigailov, Dinara Naizabayeva, Yekaterina O. Ostapchuk, Saltanat Kuatbekova, Zhaniya Dosmagambet, Moldir Kuatbek, Akerke Bissenbay, Alena Cherusheva, Akzhigit Mashzhan, Nurshat Abdolla, Sanzhar Ashimbekov, Gulnara Ismagulova, Andrey Dmitrovskiy, Seidigapbar Mamadaliyev, Yuriy Skiba
J Appl Biomed 22:115-122, 2024 | DOI: 10.32725/jab.2024.013
In 2020, there were numerous cases in Kazakhstan with clinical symptoms of COVID-19 but negative PCR results in nasopharyngeal and oropharyngeal swabs. The diagnosis was confirmed clinically and by CT scans (computed tomography). The problem with such negative PCR results for SARS-CoV-2 infection confirmation still exists and indicates the need to confirm the diagnosis in the bronchoalveolar lavage in such cases. There is also a lack of information about confirmation of SARS-CoV-2 infection in deceased patients. In this study, various tissue materials, including lungs, bronchi, and trachea, were examined from eight patients who died, presumably from...